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高效液相色谱法测定血浆中尼扎替丁浓度 预览

Determination of nizatidine by HPLC in human plasma
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摘要 目的:建立测定人血浆中尼扎替丁的高效液相色谱方法.方法:采用Diamonsil C18色谱柱(200 mm×4.6mm,5μm),流动相为0.1 mol·L-1醋酸铵缓冲液-甲醇(60:40),检测波长为320nm.血浆样品加盐析溶液碱化后以氯仿提取,雷尼替丁为内标.结果:尼扎替丁血药浓度线性范围为20~6 000l·L-1(r=0.999 9,n=6),最低检测浓度为10μg·L-1(S/N=3),方法回收率在96.84%~101.39%(n=5),日内和日间RSD均小于4%.结论:本法简便,快速,重现性好,适于尼扎替丁的药动学研究. OBJECTIVE To establish a HPLC method for the determination of nizatidine in human plasma.METHODS Nizatidine was extracted from human plasma using chloroform after basified with salting-out agent,and then separated on a Diamonsil C 18 column ( 200 mm × 4.6 mm, 5 μm).The mobile phase consisted of water( 0.1 mol · L -1 ammonium acetate)-methanol(60∶40 ). Ranitidine was used as the internal standard and the wavelength of detection was set at 320 nm.RESULTS The linear range was 20- 6 000 μg·L ...
作者 江文明 陈钧 谢月玲 高小玲 蒋新国 JIANG Wen-ming; CHEN Jun; XIE Yue-ling; GAO Xiao-ling; JIANG Xin-guo(Department of Pharmaceutics; School of Pharmacy; Fudan University; Shanghai 200 032; China);
出处 《中国医院药学杂志》 CAS CSCD 北大核心 2005年第1期,共3页 Chinese Journal of Hospital Pharmacy
关键词 尼扎替丁 高效液相色谱法 测定 nizatidine HPLC determination
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