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甘草甜素对HBsAg低表达HepG2.2.15细胞株HBV感染及TLR2、4的影响 预览 被引量:4

Effects of Glycyrrhizin on the Expression of Hepatitis B Virus and Toll Like Receptors 2,4 in HepG2.2.15 Cells Expressing Low HBsAg
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摘要 目的:探讨甘草甜素(GL)对HepG2.2.15细胞上清HBV DNA、e抗原分泌,Toll样受体2、4(TLR2、4)信号分子的表达及对细胞增殖的影响。方法:实时荧光定量PCR检测HBV DNA表达,ELISA检测HBV所分泌抗原,MTT检测细胞增殖活性,直接免疫荧光流式细胞术(FCM)检测TLR2、4在细胞株上表达的阳性细胞率,并与空白对照组对比。结果:HBsAg在该细胞株低表达,但HBeAg则明显阳性,因此研究了GL对e抗原的影响,给药后第3d e抗原总体均数间差异显著(P<0.01),但只有400μg/ml组与对照组间比较显著降低(P<0.05),800μg/ml组则较其余GL组明显升高(P<0.01)。HBV DNA给药后第3 d总体均数间比较差异有显著性,只有50μg/ml组较对照组降低,但无统计学意义(P>0.05),其余各组均较对照组升高;各组TLR2、4数值总体均数间有显著差异(P<0.01)。除200μg/ml组外,各剂量组与对照组比较显示两者均显著上调(P<0.05),但均无剂量依赖关系;MTT实验显示200μg/ml以下三个剂量组均可促进细胞增殖,但只有200μg/ml组与对照组间差异... Objective: To investigate the effects of glycyrrhizin(GL) on the expression of hepatitis B virus e antigen(HBeAg),HBV DNA,Toll-like reccptors 2,4(TLR2,4) and proliferation of cells in HepG2.2.15 cell line.Methods: Real-time PCR examined HBV DNA,ELISA examined HBsAg,HBeAg and MTT examined the proliferation of cells.FCM examined the positive percent of cells expressing TLR2,4 before and after stimulated with GL,in contrast to the blank group.Results: The expression of HBsAg was low in the cell line,so e antig...
作者 李永伟 杨宏志 柯千山 陈伟 陈雪娟 LI Yong-wei1; YANG Hong-zhi1; KE Qian-shan1; CHEN Wei2; CHEN Xue-juan3(The Third Affiliated Hospital of SUN Yat-sen University 1.Department of TCM; 2.Central Laboratory; 3.Department of Infection Disease; Guangzhou 510630; China);
出处 《中药材》 CAS CSCD 北大核心 2008年第3期,共5页 Jorunal of Chinese Medicinal Materials
关键词 甘草甜素 HBV Toll样受体2、4 HEPG2.2.15细胞株 Glycyrrhizin Hepatitis B virus TLR2 4 HepG2.2.15 cell line
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