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番木瓜ζ-胡萝卜素脱氢酶基因时空表达的荧光定量PCR分析 预览 被引量:6

Temporal-spatial Expressing Analysis of zds Gene of Papaya by Real-Time QF-PCR
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摘要 ζ-胡萝卜素脱氢酶(ZDS)是类胡萝卜素生物合成过程中的关键酶之一,它催化ζ-胡萝卜素转化成链孢红素,并催化链孢红素向番茄红素的转化。ZDS是限速酶,在植物果实颜色和花色发育过程中起着重要作用。本研究以番木瓜黄果品种Dwarf solo和红果品种Sunrise solo为材料,采用实时荧光定量PCR SYBR GreenI荧光染料法,对2个番木瓜品种的不同组织器官及果实发育成熟过程中的ZDS基因在转录水平上的表达进行了相对定量分析。结果表明,2个番木瓜品种的果实、花、叶片中均可检测到ZDS基因在转录水平上的表达,但表达量存在组织和部位上的差异。ZDS基因在成熟果实中的表达量比花中的高,花中的比叶中的高。ZDS基因在Sunrisesolo(红果肉)番木瓜果实发育成熟过程中的表达逐渐增强,且变化显著,而在Dwarf solo(黄果肉)番木瓜果实早期表达增强,成熟后期则表达趋为稳定。2个番木瓜品种成熟果肉中ZDS基因表达量存在差异,红果肉中的表达量高于黄果肉。 ζ- carotene desaturase (ZDS) which catalyzes conversion of ζ- carotene into neurosporene and then into lycopene is one of the key enzymes in the carotene biosynthesis pathway. The ZDS is a speed -limiting enzyme that plays an essential role in the process of color development in flower and fruit. A yellow fruit papaya cultivar Dwarf Solo and a red fruit cultivar Sunrise Solo were used as materials, and zds gene expression was analyzed in different organs and at five developing stages of fruits by using SYBR...
作者 高新征 言普 沈文涛 周鹏 Gao Xinzheng1; 2; Yan Pu2; Shen Wentao2; Zhou Peng1; 2 1 Hainan University; Haikou; Hainan 570228; 2 Ministry of Agriculture Key Laboratory for Biotechnology of Tropical Crops; Institute of Tropical Bioscience Biotechnology; CATAS; Hainan 571101; China;
出处 《热带作物学报》 CSCD 2009年第4期,共5页 Chinese Journal of Tropical Crops
基金 中央级事业单位基本科研业务费资助项目
关键词 番木瓜 ζ-胡萝卜素脱氢酶基因 实时荧光定量PCR 基因表达 papaya ζ- carotene desaturase gene real time QF-PCR gene expression.
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