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4株红曲霉发酵产生次级代谢产物的分析 被引量:1

Analysis of the secondary metabolites produced by 4 strains of Monascus
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摘要 目的:为研究评价红曲霉产生次级代谢产物能力,选育优良菌株。方法通过建立的发酵液中γ-氨基丁酸(GABA)、红曲色素、monacolin K 和桔霉素的比色法和高压液相色谱检测方法,比较了紫色红曲霉(Monascus purpureus)、丛毛红曲霉(Monascus pilosus)、红色红曲霉(Monascus ruber)、橙色红曲霉(Monascus aurantiacus)的液体发酵产生上述次级代谢产物能力。结果不同红曲霉菌株产生上述4种次级代谢产物的能力是不同的,4种红曲霉均可产生的红曲色素、monacolin K和桔霉素3种次级代谢产物,其中M. pilosus J2的monacolin K产量最高(8.1 mg/L)、桔霉素生成量最低(0.09 mg/L);M. purpureus Y4的红曲色素色价最高,为380 U/mL;仅有紫色红曲霉Y4可代谢产生γ-氨基丁酸,产量约为0.2 g/L。结论不同红曲霉菌株产生γ-氨基丁酸、红曲色素、monacolin K和桔霉素这4种次级代谢产物能力存在较大差异。 Objective To study the ability of secondary metabolites of Monascus, and to breed excellent strains. Methods Detection methods ofγ-aminobutyric acid (GABA), monascus pigments, monacolin K and citrinin by colorimetry and HPLC in fermentation were founded, the abilities of secondary metabolites pro-duced by Monascus purpureus, Monascus pilosus, Monascus ruber and Monascus aurantiacus were compared. Results The producing abilities of secondary metabolites among the 4 strains of Monascus were different. The four strains of Monascus could produce monascus pigments, monacolin K and citrinin. The M. pilosus strain J2 had the highest producing ability of monacolin K (8.1 mg/L) and lowest producing ability of citrinin (0.09 mg/L);M. purpureus Y4 had the highest producing ability of monascus pigments producing (380 U/mL), and only M. purpureus Y4 can produceγ-aminobutyric acid (0.2 g/L). Conclusion There are differences in producingγ-aminobutyric acid, monascus pigments, monacolin K and citrinin among different strains.
作者 张郡莹 杨强 张婵 刘国荣 王成涛 赵吉兴 ZHANG Jun-Ying[1] YANG Qiang[1] ZHANG Chan[2] LIU Guo-Rong[2] WANG Cheng-Tao[2] ZHAO Ji-Xing[3]
出处 《食品安全质量检测学报》 2014年第1期共6页 Journal of Food Safety and Quality
基金 国家自然科学基金项目,北京市自然科学基金项目,教育部科学技术研究重点项目(211101)@@@@Supported by the National Natural Science Foundation of China,Natural Science Foundation of Beijing,the Key Project of Chinese Ministry of Education
关键词 红曲霉 次级代谢产物 Γ-氨基丁酸 红曲色素 monacolin K 桔霉素 Monascus secondary metabolite γ-aminobutyric acid monascus pigments monacolin K citrinin
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