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丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究 预览 被引量:6

Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods
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摘要 采用紫外光谱和循环伏安法,研究了丁二酰化壳寡糖稀土配合物(BCS-La、BCS-Nd)与鲱鱼精DNA之间的作用方式。BCS-La、BCS-Nd的存在导致Fe(CN)3-/4-6探针分子峰电流下降,式量电位正移,表明BCSLa、BCS-Nd和探针分子与DNA之间存在竞争性作用;BCS-La和BCS-Nd分子都是通过插入方式与DNA相互作用。在一定的扫描速率范围内(0.01~0.2 V/s),在BCS-La或BCS-Nd参与的条件下,Fe(CN)3-/4-6在Au/DNA电极上的反应受吸附控制。BCS-La和BCS-Nd分别使得鲱鱼精DNA的特征峰产生明显的减色效应,最大吸收峰峰位红移,进一步表明BCS-La和BCS-Nd分别以插入方式与鲱鱼精DNA发生相互作用,导致DNA分子的构象变化。BCS-La与DNA的结合比为:n(BCS-La)∶n(DNA)=2∶1;n(BCS-Nd)∶n(DNA)=6∶1。 The interaction between succinic-oligochitosan-rare earth complexes( BCS-La and BCSNd) and herring sperm DNA was investigated by cyclic voltammetry and UV-Vis spectra. The results indicated as follows. Firstly,the peak current of probe molecule Fe( CN)3/46 at Au / DNA electrodes obviously decreased and the peak potential shifted positively because of the existences of complexes. All above these showed that there was a competitive effect between complexes and probe molecule with herring sperm DNA,which revealed that a binding mode intercalation was interacted between complexes and herring sperm DNA. The reaction of Fe( CN)3/46 at Au / DNA electrodes was controlled by adsorption mechanism in the range of scan rate from 0. 01 V / s to 0. 2 V / s. Secondly,the absorption intensity of herring sperm DNA decreased with the adding of complexes,and the maximum absorption peak had a red shift,which further illustrated that a binding mode intercalation was interacted between complexes and herring sperm DNA,thus led to the change of DNA conformation. The combining ratio of n( BCS-La) and n( BCS-Nd) with DNA was 2∶ 1 and 6∶ 1,respectively.
作者 李小芳 冯小强 杨声 王霞 LI Xiao-fang;FENG Xiao-qiang;YANG Sheng;WANG Xia;College of Life Science and Chemistry,Tianshui Normal University;Dingxi Teachers’ College;
出处 《发光学报》 EI CAS CSCD 北大核心 2013年第12期1662-1666,共5页 Chinese Journal of Luminescence
基金 天水师范学院“青蓝”人才工程资助项目
关键词 丁二酰化壳寡糖 稀土 配合物 鲱鱼精DNA succinic-oligochitosan rare earth complexes herring sperm DNA
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