Variety identification and genetic purity testing are the two important issues in vegetable seed quality control. Commonly used grow-out trials based on morphological identification are time-consuming, labor intensive and space demanding, so filed trials are difficult to distinguish the increasing varieties and test their purities. Basic principles and application of the molecular marker analysis technology, which based on the protein and genomic DNA, such as seed storage protein, isozyme, RFLP, RAPD, SSR, ISSR, AFLP in seed testing of vegetable crops are reviewed. To detect loci in parental inbred and corresponding F1 is the most important step in seed genetic purity testing of F1 hybrid. The molecular markers tightly linked with the important horticultural traits would facilitate the seed purity testing of F1 hybrid. Being objective, efficient, time-saving, less labor intensive and reproducible, the molecular marker systems would play important role in cultivars identification and seed genetic purity testing.
Molecular Plant Breeding
Molecular marker, Vegetable crops, Variety identification, Genetic purity testing