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分子标记技术在蔬菜作物品种鉴定与纯度检测中的应用 预览 被引量:22

Application of Molecular Marker in Variety Identification and Purity Testing in Vegetable Crops
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摘要 品种鉴定与遗传纯度检测是蔬菜作物种子品质检验极为重要内容.本文综述了以蛋白质、DNA为基础的分子标记,如蛋白质与同工酶、RFLP、RAPD、SSR、ISSR等分析技术应用于蔬菜种子检测的基本原理与进展.利用标记分析技术成功进行F1种子纯度检测的关键是亲本与杂交种间稳定差异位点的获得.已获得的重要园艺性状紧密连锁的遗传标记将有利于F1种子纯度鉴定.由于分子标记客观、稳定、快速、高效,这些技术将在今后种子检测中起到重要作用. Variety identification and genetic purity testing are the two important issues in vegetable seed quality control. Commonly used grow-out trials based on morphological identification are time-consuming, labor intensive and space demanding, so filed trials are difficult to distinguish the increasing varieties and test their purities. Basic principles and application of the molecular marker analysis technology, which based on the protein and genomic DNA, such as seed storage protein, isozyme, RFLP, RAPD, SSR, ISSR, AFLP in seed testing of vegetable crops are reviewed. To detect loci in parental inbred and corresponding F1 is the most important step in seed genetic purity testing of F1 hybrid. The molecular markers tightly linked with the important horticultural traits would facilitate the seed purity testing of F1 hybrid. Being objective, efficient, time-saving, less labor intensive and reproducible, the molecular marker systems would play important role in cultivars identification and seed genetic purity testing.
作者 柳李旺 侯喜林 龚义勤 张玉明 王开荣 郑军飞 Liu Liwang1* Hou Xilin1 Gong Yiqin1 Zhang Yuming2 Wang Kairong1 Zheng Junfei3 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agri .Univ., Nanjing, 2100952 Jiangsu Seed Company, Nanjing, 2100133 Shanghai Municipal Seed Company, Shanghai, 200051
出处 《分子植物育种》 CAS CSCD 2004年第4期 563-568,共6页 Molecular Plant Breeding
基金 上海市科技兴农重点攻关项目,科技部农业科技成果转化基金
关键词 分子标记技术 蔬菜作物 品种鉴定 纯度检测 Molecular marker, Vegetable crops, Variety identification, Genetic purity testing
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