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疏风通络方对哮喘大鼠嗜酸细胞跨膜迁移相关因子VCAM-1/PI3K/Rac-1/NOX2/NOX4/SHP-2表达影响的研究 预览 被引量:1

Influence of Shufeng Tongluo prescription on expression of VCAM-1/PI3K/Rac-1/NOX2/NOX4/SHP-2 related factors in eosinophils transendothelia migration in lung of rats with asthma
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摘要 [目的]探讨疏风通络方对哮喘大鼠嗜酸细胞跨膜迁移的影响。[方法]选择SPF级雄性Wistar大鼠70只为研究对象,随机分为正常组、模型组、地塞米松组、DPI组、中药低、中、高剂量组,共7组,每组大鼠10只。第1天致敏:正常组给予生理盐水1 mL腹腔注射致敏,而其他6组给予1 mL造模液(含V级卵清蛋白100 mg,氢氧化铝100 mg和灭活百日咳杆菌6×109个)腹腔注射致敏;第15天开始激发:将各组大鼠分别置于相同大小的雾化箱内,正常组给予生理盐水6 mL雾化激发,其他6组给予5%的V级卵清蛋白溶液6 mL雾化激发,每次激发前2 h正常组给予生理盐水1 mL灌胃,而其他6组给予相应的药物灌胃。各组每天激发1次,每次激发30 min,连续激发10 d后处死大鼠,并采集相应标本,并进一步检测影响嗜酸细胞跨膜迁移的相关指标。[结果]模型组肺组织嗜酸细胞跨膜迁移相关调节因子血管内皮黏附分子-1(VCAM-1)、磷脂酰肌醇3激酶(PI3K)、Rac-1、NADPH氧化酶2(NOX2)、NADPH氧化酶4(NOX4)、酪氨酸磷酸酶(SHP-2)出现高表达,高于正常组(P【0.001);各治疗组与模型组相比,地塞米松组抑制上述因子表达的效果最好(P【0.001),中药高剂量组的抑制效果略差于地塞米松组,但两者差异无统计学意义(P】0.05),氯化二联苯碘(DPI)组、中药低、中剂量组抑制效果一般。[结论]疏风通络方对哮喘大鼠嗜酸细胞跨膜迁移有一定的阻抑作用,这可能是其平喘作用机制之一。 [Objective] To explore the effect of Shufeng Tongluo decoction on eosinophils transendothelia migration in lung of rats with asthma.[Methods] Seventy Wistar rats(SPF) were randomly divided into seven groups: normal group,model group,dexamethasone group,DPI group,low-dose group of Chinese medicine,medium-dose group of Chinese medicine,and high-dose group of Chinese medicine with 10 rats in each group.The rats in normal group were sensitized by 1 mL of intra-peritoneal injection of normal saline.The other 6 groups were given with 1 mL sensitization liquid,contained 100 mg OVA(Grade V),100 mg aluminum hydroxide and 6×109 inactivated whooping cough bacili on the first day of experimental study.From 15th day,the rats in normal group were administrated with 1 mL of normal saline orally,2 hours later the rats were challenged by 6 mL normal saline in a box of aerosol inhalation once a day for 30 min a time and keeping for 10 days;the rats in other 6 group were respectively administrated with corresponding drugs orally and 2 hours later were challenged by 6 mL OVA(5%,V Grade) of aerosol inhalation once a day,for 30 min a time and keeping for 10 days,And 10 days later all rats were killed.The samples from rats were collected for examination.[Results] The expression of VCAM-1/PI3K/Rac-1/NOX2/NOX4/SHP-2 related factors to eosinophils transendothelia migration in lung of rat with asthma were significantly higher than that of normal group rats(P<0.001).Compared with the model group,dexamethasone group could significantly inhibit the expression of above factors(P<0.001).The effect of high-dose group of Chinese medicine was similar to dexamethasone group(P>0.05),the other treatment groups(DPI group,low-dose group of Chinese medicine and medium-dose group of Chinese medicine) only had general inhibition effect.[Conclusion] Shufeng Tongluo decoction has some certain suppression effect on eosinophils transendothelia migration in lung of rats with asthma to a certain extent.It may be one of its action mechanisms in controlling asth
作者 刘贵颖 张慧琪 尹新中 朱振刚 刘旻 乔亚珍 窦迎婷 刘文彬 刘超武 狄冠麟 常力 田常宏 LIU Gui-ying,ZHANG Hui-qi,YIN Xin-zhong,ZHU Zhen-gang,LIU Min,QIAO Ya-zhen,DOU Ying-ting, LIU Wen-bin,LIU Chao-wu,DI Guan-lin,CHANG Li,TIAN Chang-hong(Respiratory Department,The First Affilated Hospital of Tianjin University of TCM,Tianjin 300193,China)
出处 《天津中医药》 CAS 2013年第3期159-163,共5页 Tianjin Journal of Traditional Chin Medicine
基金 国家自然科学基金面上项目(30973727)
关键词 支气管哮喘 嗜酸细胞 跨膜迁移 疏风通络方 基因表达 asthma eosinophils transmembrane migration Shufeng Tongluo prescription gene expression
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参考文献12

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