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神经根慢性嵌压损伤的动物模型建立 被引量:6

The animal model of chronic compressive injury of spinal nerve roots
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摘要 目的:建立一种由自身骨性增生造成神经根慢性嵌压损伤的动物模型,为相关实验提供造模方法.方法:30只健康家猫,手术显露右侧C7、C8和L5、L6神经根及其椎间孔内口,用牙髓钻破坏椎间孔周围骨皮质后,将'V'形松质骨块沿骨壁嵌于神经根通道的骨性管道内及侧隐窝后方,左侧做正常对照.在造模术前和术后第2、4、8、12、24周行磁刺激运动诱发电位(MEP)检测,每次随机选6只,4~5只行影像学检查,以确定神经根通道的狭窄程度和神经根受压状态,6只均做病理组织学检查和椎间孔截面积测量.结果:术后早期实验侧肢体出现行为异常;而后有不同程度肌萎缩;后期部分肢体远端出现溃疡.影像学检查随着嵌压时间延长,实验侧椎间孔骨痂增多,狭窄加重,神经根受压变形,椎间孔骨性截面积8周后明显减小,与对照侧比较有显著性差异.术后2周,神经根组织学检查主要表现为神经束膜、内膜的水肿,髓鞘肿胀;4周后发生节段性脱髓鞘;8周时神经轴突增粗、断裂,远端瓦勒氏变性;12周后变性神经结构崩解、吸收,形成空洞;24周时整个神经干纤维化.术后4周时实验侧MEP开始出现潜伏期延长;8周时伴有波形分化不清;12周波幅明显下降;24周MEP的引出困难,部分电位消失.结论:采用椎间孔内自体松质骨植入,造成神经根慢性嵌压性损伤模型成功率高,操作简单,适用于脊柱各个节段,其损伤部位和形式与临床更为接近. Objectives:To set up an experimental animal model of spinal nerve root chronic compression in-jury caused by self-bone hyperplasia,so as to provide a model-making method for relevant experiments.Method:30healthy cats with weight between3to5kilograms and sex at random.After having exposed the nerve roots of right side and the intervertebral foramina,transplanting autogenous cancellous bone to the nerve root pathway.Left side was for contral.MEP examinations were taken before the operation and at the2nd,4th,8th,12th and24th weeks after the operation.Every time6cats were chosen at random for neuropathology ex-amination and intervertebral foramina section area measurement.At the same time,4to5cats were examined by X-ray,CT and MRI to determine the narrowness of the nerve roots pathway and the situation of compres-sion.Result:Abnormal behavior of extremity on experimental side occurred in early post-operation,followed by muscular atrophy to different extent.In late post-operation,ulcer appeared at the distal limbs.With prolongation of the compression period,increased osteotylus occurred in the intervertebral foramina of the experimental side which became narrower,and nerve roots were deformed due to compression.The section area was reduced sig-nificantly8weeks later with significant difference from the contrast side.The main changes of the nerve root were the edema of perineurium?endoneurium and myelin swelling.myelin decorticated segmentally4weeks lat-er.Nerve axon became thicker and broken,peripheral Wallerian degeneration8weeks later.12weeks later,the degenerated never structure destroyed and absorbed,followed by the cavities formation.The whole nerve became fibrosis in24weeks.4weeks after operation,incubation period of MEP on experimental side started to pro-long,differentiation of wave shape was unclear at the8th week,amplitude dropped at the12th week,and24weeks later,there were difficulties in MEP induction and part potential disappeared.Conclusion:Making a mod-el of nerve root chronic injury by transplanting au
作者 杨大志 王坤正 陈君长 镇万新 王多 YANG Dazhi,WANG Kunzheng,CHEN J unchang,et al[Author’s address]The Department of Spinal Surgery,the2nd Affiliated Hospital of Ji’nan University Medi-cal School,Shenzhen,518020,China
出处 《中国脊柱脊髓杂志》 CAS CSCD 2004年第5期 290-294,F003,共6页 Chinese Journal of Spine and Spinal Cord
关键词 神经根 慢性损伤 动物模型 Nerve root Chronic injury Animal model Cat
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参考文献9

  • 1潘映辐主编..临床诱发电位学 第2版[M].北京:人民卫生出版社,1988:752.
  • 2岳寿伟,吴宗耀,徐淑军,刘德山,王丹影,王永惠.兔腰神经根慢性压迫模型的建立[J].中国康复医学杂志,2002,17(4):205-207. 被引量:9
  • 3Carlstedt T, Risling M. Expression of neuregulin and ErbB3and ErbB4 after a traumatic lesion in the ventral funiculus of the spinal cord and in the intact primary offactory system [J].Exp Brain Res,2002,142( 1 ):81-90. 被引量:1
  • 4Bergmark M,Kanje M,Widerberg A,et al. Experimental nerve compression and upregulation of CPON in DRG[J]. Neuroreport, 2001,12 ( 17):3783-3786. 被引量:1
  • 5Omefjord M,Olmarker K,Otani K,et al. Effects of diclofenac and ketoprofen on nerve conduction velocity in experimental nerve root compression[J].Spine,2001,26(20):2193-2197. 被引量:1
  • 6Shinichi Kikuchi, Shinichi Konno,Satoru Kayama. Inereased resistance to acute compression injury in chronically compressed spinal nerve root[J].Spine, 1996,21 (22):2544-2550. 被引量:1
  • 7Michael C, Katsuhiko S,Kjell O,et al. A model for chronic nerve root compression studies[J].Spine, 1997,22 (9) :946-957. 被引量:1
  • 8Yoshizawa H,Kobayashi S,Morita T,et al. Chronic nerve root compression:pathopysiologic mechanism of nerve root dysfunction[J].Spine, 1999,24 (2): 194-199. 被引量:1
  • 9汤晓芙.神经肌肉疾病的电生理诊断原理及应用 Ⅰ.原理概述[J].中华神经精神科杂志,1993,26(5):305-307. 被引量:2

二级参考文献1

  • 1周耀 廖维洪 等.脊神经节损失的组织形态与电生理观察[J].中国修复重建外科杂志,1998,12(3):145-149. 被引量:3

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