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益气解毒颗粒干预鼻咽癌细胞HNE1蛋白质表达的研究 预览 被引量:13

An investigation on the proteomics of nasopharyngeal carcinoma cell line HNE1 treated with Qi-boosting toxin-resolving granule
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摘要 目的探讨中药复方益气解毒颗粒对鼻咽癌细胞杀伤作用的分子机制.方法筛选益气解毒颗粒药液对鼻咽癌杀伤的半抑制浓度(IC50),用0.1倍半抑制浓度(0.1×IC50)的益气解毒颗粒作用鼻咽癌细胞HNEl 96h,用固相pH梯度双向凝胶电泳分离益气解毒颗粒处理和未处理的鼻咽癌细胞HNE1的总蛋白质,银染显色,PDQuest 2-de软件分析差异蛋白质点.结果益气解毒颗粒药液杀伤鼻咽癌细胞HNE1的半抑制浓度为6.25mg/ml;双向电泳图像分析益气解毒颗粒药物处理组图像的平均蛋白质数为1120±89,未处理组的平均蛋白质数为1281±102;差异蛋白质有673个,其中有218个表达增强,有455个蛋白质表达下降.结论益气解毒颗粒对鼻咽癌细胞的杀伤作用这些差异表达蛋白质有关,这些差异蛋白质有待质谱分析鉴定,为中药作用的分子机理研究提供了新的思路. Objective To investigate the molecular mochanism of Qi-Boosting Toxin Resolving Granule (QBTRG)in the inhibition of nasopharyngeal carcinoma cell line HNE1 based on the techniques of proteomics. Methods The IC_(50) of QBTRG killing HNE1 cells was determined by MTT at first. Then, HNE1 cells were treated with QBTRG at the concentration of 0.1×IC_(50) for 96 hours. After this step, the cells were collected and the total protein was extracted from these cells. By two-dimension clec- trophoresis, the proteins were separated and protein profiles were obtained. Followed was an analysis on the differently expressed proteins by PDQuest2-de microsolft on the basis of protein profile comparison between two groups of HNE1 cells treated with QB- TRG and those not treated at all. Results The IC_(50) of QBTRG on NHE1 cells was 6.25mg/ml. The mean number of protein spots was 1120±89 for treated HNE1 cells with QBTRG and that was 1281±102 for the untreated HNE1 cells. Therefore, the dif- ferently expressed protein spots were 673, among them 218 being up-regulated and 455 being down-regulated in their expression in treated HNE1 cells. Conclusions The in- hibitory effects of QBTRG on nasopharyngeal careinoma cells may be closely associated with the pattern of these differently expressed proteins. The physical and chemical prop- erties of these proteins can be identified by the means of MS. Then, the data got from this identification should be helpful for the further investigation on the molecular mecha- nism of QBTRG in the inhibition of this kind of cancer cells.
作者 唐发清 田道法 龚志军 顾焕华 荆照政 肖志强 唐银 TANG Faqing;TIAN Daofa;GONG Zhijun;GU Huanhua;JING Zhaozheng;XIAO Zhiqiang;TANG Yin Dept. of Otolaryngology, Xiangya Hospital, Central South University, Changsha 410078
出处 《中国中西医结合耳鼻咽喉科杂志》 2004年第3期 120-123,共4页 Chinese Journal of Otorhinolaryngology of Integrated Traditional and Western Medicine
基金 国家自然科学基金,国家中医药管理局科研项目,湖南省教育厅科研项目
关键词 益气解毒颗粒 鼻咽癌 细胞HNE1蛋白质 表达 双向电泳 Nasopharyngeal carcinoma Proteomics Two-dimensional electrophoresis Qi-Boosting Toxin-Resolving Granule
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参考文献9

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