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水分胁迫对抗旱性不同的冬小麦品种叶片蛋白质影响的比较 预览 被引量:18

A Comparison of the Effect of Water Stress on Proteins in Leaves of Drought-Tolerant and Drought-Sensitive Cultivars of Winter Wheat
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摘要 选用两种抗旱性不同的冬小麦-太原633(抗旱)和C609(干旱敏感),研究了水分胁迫条件下植物蛋白质代射变化的规律及与植物抗旱性的关系。双向电泳结果表明,-2.0MPa PEG-6000水分胁迫引起了两品种冬小麦叶片蛋白质变化的明显差异。抗旱品种在水分胁迫时蛋白质代射变化的时间顺序。干旱诱导的蛋白质的变化包括干旱诱导蛋白及蛋白质含量的在复水后均可迅速恢复;蛋白质转录抑制剂-放线菌素D可抑制干旱诱导 Two winter wheat cultivars differing in drought resistance (Taiyuan 633, drought-tolerant; C609, drought-sensitive) were used to study the changes in protein synthesis and the relation between these changes and drought resistance of plants under the water stress. The results of two-dimensional PAGE-SDS-PAGE electrophoresis showed that water stress(-2.0MPa) caused obvious differences of protein changes in leaves of two winter wheat cultivars. The water-stress-induced protein in Tai Yuan 633 presented earlier than that in C609. The results indicated that there was a close connection between the proteinchanges in drought-tolerant and drought sensitive cultivars caused by water stress and thetime of water stress. After rewatering the protein changes could recover immediatly. The experiment of using the inhibitor showed that the emergence of the water-stress-induced proteins could be inhibited. The results implied that the gene expression of the proteins relating to adaptation to water stress was regulated mainly at transcriptional level.
作者 李霞 李云荫 Li Xia Li Yunyin Cao Min(Department of Biology, Hebei Normal University, Shijiazhuang 050016)
出处 《华北农学报》 CSCD 北大核心 1993年第4期 20-25,共6页 Acta Agriculturae Boreali-Sinica
关键词 小麦 水分胁迫 蛋白 叶片 品种 Winter wheat Leaf Water stress Protein
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  • 1L. C. Loon. Pathogenesis-related proteins[J] 1985,Plant Molecular Biology(2-3):111~116 被引量:1
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  • 3L. Pitto,F. Lo Schiavo,G. Giuliano,M. Terzi. Analysis of the heat-shock protein pattern during somatic embryogenesis of carrot[J] 1983,Plant Molecular Biology(5):231~237 被引量:1

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