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口虾蛄提取物对人鼻咽癌细胞基质金属蛋白酶-9的抑制作用 预览 被引量:10

Inhibition effect of Oratosquilla extract on human nasopharyngeal carcinoma cell matrix metal protease-9
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摘要 目的探讨口虾蛄提取物对CNE-2Z细胞基质金属蛋白酶-9(MMP-9)的作用.方法采用细胞免疫化学法及明胶酶谱法检测药物对CNE-2Z细胞MMP-9表达及分泌的影响;体外侵袭试验检测药物对CNE-2Z细胞侵袭能力的影响.结果口虾蛄提取物能抑制CNE-2Z细胞MMP-9表达及分泌,以量效方式抑制CNE-2Z细胞体外侵袭能力.结论口虾蛄提取物能通过抑制MMP-9表达及分泌而降低CNE-2Z细胞体外侵袭能力. Objective It is to discuss the action of the Oratosquilla extract on human nasopharyngeal carcinoma (CNE-2Z) cell matrix metal protease-9 (MMP-9).Methods Immunocytochemistroy and gelatin zymography were used to study the effects of the drug on the expression and secretion of CNE-2Z cell MMP-9. Invasion test in vitro was adopted to detect the influence of the drug on the invasion ability of CNE-2Z cell.Results The Oratosquilla extract could inhibit the expression and secretion of CNE-2Z cell MMP-9, and inhibit the invasion ability in vitro of CNE-2Z with dose-effect fashion.Conclusion The Oratosquilla extract can reduce the invasion ability in vitro of CNE-2Z through inhibiting the expression and secretion of MMP-9.
作者 顾帝水 孔霞 黄培春 GU Di-shui, KONG Xia, HUANG Pei-chun (Guangdong Medical College, Zhanjiang 524023, Guangdong, China)
机构地区 广东医学院
出处 《现代中西医结合杂志》 CAS 2004年第21期 2816-2818,共3页 Modern Journal of Integrated Chinese Traditional and Western Medicine
关键词 CNE-2Z细胞 MMP-9 体外侵袭 提取物 基质金属蛋白酶-9 表达 鼻咽癌细胞 降低 抑制 药物 nasopharyngeal neoplasm matrix metal protease Oratosquilla
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