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家蝇幼虫天蚕素基因的克隆与序列分析 预览 被引量:13

Cloning and sequence analysis of the cDNA encoding cecropin an antimicrobial peptide from Musca domestica larvae
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摘要 目的对家蝇幼虫天蚕素基因进行克隆和序列分析.方法根据Genbank公布的家蝇成虫天蚕素Cecropin基因序列设计一对引物,以家蝇幼虫cDNA文库液为模板,PCR扩增该基因的编码区序列.结果家蝇幼虫天蚕素cDNA目的基因的长度为229bp,与成虫防御素基因一致性为96%;最大的ORF位于20bp~211bp,含192bp,编码63个氨基酸.结论初步预测了该基因编码蛋白的化学性质和二级结构,为该基因的重组表达研究打下基础. Objective To clone the cecropin gene of Musca domestica larvae and analyze its sequence. Methods A pair of primer were designed based on published cecropin sequence of adult Musca domestica on Genbank taken cDNA library of Munsca domestica larvae as template and the cDNA encoding cecropin, an antimicrobial peptide from Musca domestica was amplified by PCR. Results The size of product of cDNA of Musca domestica larvae was 229 bp and its coincidence rate with adult Musca domestica phylaxin gene was 96%. The maximum ORF was located at 20bp~211 bp, containing a192bp and encoding 63 amino acids. Conclusion The chemical nature and dimentional structure of the cDNA encoding the gene was preliminarily analyzed to have set basis for the researches of recombinant expression of this cDNA.
作者 金小宝 许琴英 徐建华 朱家勇 JIN Xiao-bao1, XU Qin-ying2, XU Jian-hua2, et al. ( Department of Parasitology of Basic School of Guangdong Pharmaceutical College, Guangzhou 510224, Guangdong, P. R. China )
出处 《中国热带医学》 CAS 2004年第6期 903-906,共4页 China Tropical Medicine
基金 广东省科技厅科技计划,广东省广州市科技局科技攻关项目
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  • 1胡云龙,赵学忠.抗菌肽的分子生物学研究进展[J].生物工程进展,1997,17(3):14-18. 被引量:32
  • 2[2]Jiang G, Harrison DJ.mRNA isolation in a microfluidic device for eventual integration of cDNA library construction[J]. Analyst, 2000,125(12) :2176~ 2179. 被引量:1
  • 3[3]Derubeis AR, Young MF,Fisher LW, et al. Double FYVE-containing protein 1 (DFCP1): isolation, cloning and characterization of a novel FYVE finger protein from a human bone marrow cDNA library[J]. Gene, 2000,255 (2): 195 ~ 203. 被引量:1
  • 4[4]Luo LY,Soosaipillai A, Diamandis EP. Molecular cloning of a novel human gene on chromosome 4pll by immunoscreening of an ovarian carcinoma cDNA library[J]. Biochem-Biophys-Res-commun,2001,280(1) :401 ~ 406. 被引量:1
  • 5[5]Watanabe J,Sasaki M, Sugano S.FULL- malaria: a database for a full -length enriched cDNA library from human malaria parasite, Plssmodium falciparum[ J]. Nucleic - Acids - Res,2001,29( 1 ) :70 ~ 71. 被引量:1
  • 6[6]Sakai R, Kinouchi T, Yamagami S, et al. Construction of human corneal endothelial cDNA library and identification of novel active genes[J]. InvestOphthalmol-Vis-Sci,2002,43(6): 1749 ~ 56. 被引量:1
  • 7[7]Boman HG, Faye I, Gudmundsson GH, et al. Fed Eur Biochem Soc Letters, 1989,259:103 ~ 106. 被引量:1
  • 8[8]Clark JM.Novel non - templated nucleotide adittion reations catalyzed by procaryotic and eucaryotic DNA polymerase[J].Nucleic Acids Res, 1988,16:9677~9686. 被引量:1
  • 9[9]Mead DA, Pey NK, Hernstadt C, et al.A universal method for the direct cloning of PCR amplified nucleic acid[J]. Biotechnology, 1991,9:657 ~663. 被引量:1
  • 10[10]Marchuk D, Drumm M, Saulino A, et al. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products[J].Nucleic Acids Res, 1991,19:1154 ~ 1157. 被引量:1

二级参考文献5

  • 1韩献萍,生物技术通讯,1994年,5卷,1期 被引量:1
  • 2夏明明,博士学位论文,1993年 被引量:1
  • 3戴祝英,江苏省生物技术学术讨论会论文集,1990年 被引量:1
  • 4屈贤铭,生物化学与生物物理学报,1989年,21卷,35页 被引量:1
  • 5张双全,生物化学杂志,1987年,3卷,1期,11页 被引量:1

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