Objective To clone the cecropin gene of Musca domestica larvae and analyze its sequence. Methods A pair of primer were designed based on published cecropin sequence of adult Musca domestica on Genbank taken cDNA library of Munsca domestica larvae as template and the cDNA encoding cecropin, an antimicrobial peptide from Musca domestica was amplified by PCR. Results The size of product of cDNA of Musca domestica larvae was 229 bp and its coincidence rate with adult Musca domestica phylaxin gene was 96%. The maximum ORF was located at 20bp～211 bp, containing a192bp and encoding 63 amino acids. Conclusion The chemical nature and dimentional structure of the cDNA encoding the gene was preliminarily analyzed to have set basis for the researches of recombinant expression of this cDNA.
China Tropical Medicine