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拟南芥黑芥子酶基因根特异性表达启动子的克隆 预览 被引量:7

Cloning of root specific expression promoter of myrosinase ( pyk10 ) gene from Arabidopsis thaliana
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摘要 黑芥子酶是一类催化芥子油苷水解的同工酶,pyk10是一个在拟南芥根和下胚轴中特异表达的黑芥子酶基因.本研究用PCR方法从拟南芥Columbia生态型基因组中克隆了pyk10基因启动子片段.序列分析表明:该片段全长1 454 bp,与已发表的pyk10启动子序列(GenBank accession no.AJ292756)同源性达98%.该启动子片段中含有多种其他植物基因启动子中发现的通用启动子元件,并含有器官和组织特异性转录因子的结合位点ACGT-、CANNTG-、GATA-以及Ⅰ盒等顺式元件. Myrosinase are a group of isoenzymes catalyzing the hydrolysis of glucosinolates. pyk10 is a root and hypocotyls specific myrosinase gene from Arabidopsis thaliana. The promoter fragment of pyk10 was cloned by PCR method from Arabidopsis thaliana genomic DNA. Nucleotide sequence analysis showed that the fragment cloned was consist of 1 454 bp. Comparison the sequence obtained with that reported (GenBank Accession no. AJ292756) showed that the nucleotide homology was as high as 98%. The pyk10 promoter contains regulatory sequence elements that have shown to be functional cis-sequences in other plant promoters, such as ACGT-,CANNTG-,GATA and I-box- cis-elements, which are binding sites for organ and tissue-specific transcription factors.
作者 李桂兰 王文颇 臧少先 乔亚科 杨少辉 薄涛 李明刚 LI Gui-lan~1,WANG Wen-po~1,ZANG Shao-xian~2,QIAO Ya-ke~1,YANG Shao-hui~3,BO Tao~3,LI Ming-gang~3(1. Hebei Normal University of Science & Technology, Changli 066600, China; 2. Baoding Vocation-technical College, Baoding 071001, China;3. Institute for Molecular Biology, Nankai University, Tianjin 300071, China)
出处 《河北农业大学学报》 CAS CSCD 北大核心 2005年第1期 49-52,共4页 Journal of Agricultural University of Hebei
基金 河北省自然科学基金,河北省科技攻关项目,河北科技师范学院校科研和教改项目
关键词 拟南芥 pyk10 根特异性 启动子 Arabidopsis thaliana pyk10 root specific promoter
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