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氧化苦参碱对放疗鼻咽癌HNE-1细胞P-糖蛋白表达影响 预览 被引量:11

Effects of oxymartine on expression of P-glycoprotein in HNE-1 cell line radiation therapy
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摘要 目的:(1)氧化苦参碱对放射前后鼻咽癌HNE-1细胞有无生长抑制作用;(2)研究天然产物氧化苦参碱对鼻咽癌HNE-1细胞株放射前、后P-糖蛋白表达的影响.方法:(1)MTT法检测长春新碱、维拉帕米、氧化苦参碱对放射前HNE-1细胞、放射后HNE 1(200)细胞生长抑制情况;(2)免疫化学法检测氧化苦参碱、维拉帕米对HNE-1(200)细胞P糖蛋白表达的影响.结果:(1)长春新碱、维拉帕米、氧化苦参碱对放射前、后细胞生长均有抑制作用,呈浓度依赖关系,随着浓度的增加抑制作用也增加;(2)高浓度VCR对放射治疗后细胞HNE-1(200)的抑制率比对HNE-1细胞的抑制率降低了近50%,P值小于0.01,有显著差异;氧化苦参碱、维拉帕米对两种实验细胞抑制没有显著差异.(3)免疫组化检测细胞P-糖蛋白表达,结果为HNE-1阴性,HNE-1(200)阳性,维拉帕米、氧化苦参碱分别作用于HNE-1(200)细胞后P-gp表达弱阳性.结论:(1)氧化苦参碱对放射前后鼻咽癌HNE-1细胞有明显生长抑制作用;(2)放射后细胞HNE-1(200)对长春新碱产生耐药;(3)低毒性浓度氧化苦参碱具有与维拉帕米相似的作用,能使放射照射后鼻咽癌HNE-1细胞株P-糖蛋白的表达下降. Objective:To investigate the effects of oxymartine on cell growth and the changes of P-glycoprotein in HNE-1 cell line after exposed to fractionated X-irradiation.Methods:By MTT,the cell proliferation was observed both before X-irradiation and after X-irradiation conditions with the extent of vincristine,oxymartine and verapamil respectively.P-glycoprotein expression was tested by immunohistochemistory method SP.Results:(1)Vincristine,oxymartine and verapamil were capable of inhibiting cell growth on HNE-1 cell line and fractionated X-irradiated HNE-1(200)cell subline;(2)Vincristine was less inhibitory on cell growth in HNE-1(200)cell subline than HNE-1 cell line,and the difference had statistic significance;(3)P-glycoprotein expression in HNE-1 cell line was negative while HNE-1(200)cell subline positive;oxymartine with low inhibitory concentration could increase the expression of P-glycoprotein in HNE-1(200) cell subline,which is similar to that of verapamil.Conclusion:(1)Oxymartine is capable of inhibiting cell growth on HNE-1 cell line and fractionated X-irradiated HNE-1(200)cell subline;(2)HNE-I(200)cell subline is resistant to vincristine;(3)Oxymartine with low inhibitory concentration could increase the expression of P-glycoprotein in HNE-1(200)cell subline.which is similar to that of verapamil.
作者 王驰 陈鸿雁 舒艳 邱宗荫 WANG Chi,et al (Department of Organic Chemistry,College of Basic Medicine,Chongqing Medical University)
出处 《重庆医科大学学报》 CAS CSCD 2005年第1期 16-19,共4页 Journal of Chongqing Medical University
基金 重庆市卫生局中医药科研项目
关键词 鼻咽癌FINE-1细胞株 P-糖蛋白 MTT法 氧化苦参碱 HNE-1 cell line P-glycoprotein MTT Oxymartine
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