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肉桂醛和柠檬醛对黄曲霉及烟曲霉细胞DNA与RNA的影响 预览 被引量:24

Effect of cinnamaldehyde and citral on DNA and RNA in Aspergillus flavus and A. fumigatus cells
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摘要 目的研究肉桂醛、柠檬醛对黄曲霉和烟曲霉细胞DNA、RNA水平的影响,以期探明其抗真菌的作用机制.方法黄曲霉、烟曲霉接种在含有不同浓度药物的蔡氏固体培养基中,同时设不加药物为空白对照,置26.5℃恒温培养3~6 d,将对照组和用药组真菌细胞进行激光扫描共聚焦显微镜观察并作细胞扫描图像分析,以对DNA、RNA进行定量和定位观察.结果用药组DNA、RNA水平发生不同变化,出现多核分生孢子.结论肉桂醛、柠檬醛直接或间接影响了真菌细胞遗传物质的正常合成,以至不能完成正常细胞周期,影响分生孢子梗的正常分化,从而抑制真菌生长、繁殖. Objective To investigate the effects of cinnamaldehyde and citral on DNA and RNA of Aspergillus flavus and A. fumigatus cells and their mechanisms. Methods A. flavus and A. fumigatus were incubated on Czapeks agar plate (treated with cinnamaldehyde and citral at different concentrations) at 26.5 ℃ for 3—6 d. The normal and treated cells were observed by laser scanning confocal microscope (LSCM) and image analysis to describe the DNA and RNA levels by quantity and localization. Results DNA and RNA levels were changed greatly and multinucleate coniospores appeared in the treated cells. Conclusion Cinnamaldehyde and citral have directly or indirectly interfered the conventional synthesis of fungal hereditary DNA and RNA and normal differentiation of conidiophore in A. flavus and A. fumigatus, thus inhibiting the normal cell cycle and the growth and propagation of fungi.
作者 谢小梅 龙凯 许杨 方建茹 XIE Xiao-mei1, LONG Kai2, XU Yang1, FANG Jian-ru2 (1.The Key Laboratory of Food Science of MOE, Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China; 2. Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, China)
出处 《中草药》 CAS CSCD 北大核心 2005年第4期 558-560,共3页 Chinese Traditional and Herbal Drugs
基金 国家自然科学基金
关键词 肉桂醛 柠檬醛 黄曲霉 烟曲霉 DNA RNA 激光扫描共聚焦显微镜 cinnamaldehyde citral Aspergillus flavus Link A. fumigatus Fres. DNA RNA laser scanning confocal microscope (LSCM)
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