The purpose was to study the responses of AML cell treated with cytochrome C and to explore the influence of cytochrome C on apoptosis of AML cell induced by daunorudicine (DNR). The differentiation of AML cell was detected by Wright-Giemsa staining and NBT test, the apoptosis of AML cell was assayed by flow cytometry and fluorescence microscopy. The results showed as follows: (1) different concentrations of cytochrome C could induce different effects on AML cells. Concentration of cytochrome C for differentiation was 10 μ l/ml, for apoptosis was 20 μ l/ml, and for necrosis was 40 μ l/ml. ( 2) the apoptosis of AML cells decreased with the administration of cytochrome C in 10.0 μ lg/ml before treating AML cells with DNR ( P <0.01), but no change was shown with the administration of cytochrome C in 20.0 μ l/ml ( P >0.05). (3) in reverse sequence, administrating of cytochrome C in 10 μ l/ml and 20 μ l/ml after treating AML cells with DNR, two different concentrations of cytochrome C could increase the apoptosis of AML cells ( P <0.01). It is suggested that cytochrome C may probably affect the apoptosis of AML cells induced by DNR.
Journal of Experimental Hematology