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生物农药麦丰宁B3对小麦纹枯病菌的抑制作用 预览 被引量:37

INHIBITION OF MAIFENGNING B_3(BACILLUS SP. ,A BIOLOGICAL FUNGICIDE) TO WHEAT SHARP EYESPOT (RHIZOCTONIA CEREALIS)
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摘要 用平板双培养法在PDA,NA及KB3种培养基上测定B3菌株对小麦纹枯病菌均有显著抑制作用,用喷菌法,四周划线法和四角点菌法测定的抑菌效果为73.6%~99.5%。B3的无细胞培养滤液也明显著抑制病菌菌丝生长,菌核形成和菌核萌发。PDA中含12.5%B3滤液时对菌丝生长的抑制效果达80%以上。滤液含量为10%时,病菌不能形成菌核。B3无细胞培养滤液可使含病菌菌丝溶液电导率上升,还原糖,氨基酸和蛋白质 B3(Bacillus sp. ) showed powerful inhibition to a wheat pathogen RC-54 (R.cerealis) on 3 media (PDA,NA and K'B),with an effectiveness of 73. 7%~99. 5% when tested with different methods. Cell-free culture filtrate (CFCF) of B3 could also inhibit mycelial growth of RC-54. with an efficiency of 80% at the dosage of 12. 5%. On PDA medium containing B3-CFCF, RC-54 needed more days to form sclerotia, and produced a few scle-rotia. When B3-CFCF was increased to 10%,no sclerotia was formed. And further more, germination of sclerotia was also inhibited by B3-CFCF. Addition of B3-CFCF to NB or steriled H2O with RC-54 mycelia could increase the conductance and infiltration of reducing sugars, amino acids and proteins. Heated B3-CFCF showed reduced inhibition. Microscopy revealed abnormal, more-branching growth of RC-54 treated with B3-CFCF.
作者 张学君 凌宏通 李洪连 王金生 Zhang Xuejun ling Hongtong Li Honglian Wang Jinsheng (Dept. of Plant Protection, Nanjing Agricultural University, Nanjing 210014)
出处 《植物病理学报》 CAS CSCD 北大核心 1994年第4期 361-366,共6页 Acta Phytopathologica Sinica
基金 国家“八五”攻关课题 85-10-02-04-01资助
关键词 小麦 纹枯病 生物农药 麦丰宁B3 Wheat sharp eyespot Biological fungicide Maifengning B_3(Bacillus sp. )
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参考文献3

  • 1张志良,植物生物化学技术和方法,1986年 被引量:1
  • 2张龙翔,生化实验方法和技术,1981年 被引量:1
  • 3荆家海,植物生物化学分析方法,1981年 被引量:1

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