Objective:Using the method of partial portal vei n ligation (PVL) to develop the experimental model of Portal hypertensive gastropa thy(PHG), from content of ET, to study the principle of PHG and the prevention a nd cure effect of the prescription of smoothing the liver-Qi, acting blood and strengthening the spleen.Methods:120Wistar male rats were divided into eight gro ups randomly- Using the method of partial portal vein ligation (PVL) to develop the experimental model of Portal hypertensive gastropathy(PHG).The rats of ever y groups were got the stomach tissues to test Endothelin (ET) and observed with pathology. Results: The average B-mode titre of ET of group A in gastric fund us tissue are significantly higher than group F using immunohistochemical method (P<0.01). The average B-mode titre of ET of group B、C、D in gastric fundus tissue are significantly higher than group E using immunohistochemical m ethod(P<0.01) The effect of group B is more excellent than group D (P< 0.05). There are many pathologic changes in stomach tissues ,such as arterio -venous shunt formation, communicating branches increasing, microvessel expandi ng and distorted with group A、B、C、D、E、F. But the severity of stomach mucous membrane damages in group E and F were more obvious than other groups, the seve rity of stomach mucous membrane damages in group B is the most light.Conclusion: ET has an close relation with the principle of PHG. The prescription of smoothin g the liver-Qi, acting blood and strengthening the spleen has positive effects in preventing and curing PHG.
Journal of Hubei College of Traditional Chinese Medicine