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PSY基因对大豆的遗传转化 预览 被引量:2

Genetic Transformation of Gene (PSY) into Soybean
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摘要 以3个大豆品种的体细胞胚为受体,以PSY为目的基因,应用基因枪法对大豆进行了遗传转化,经大豆体细胞胚的继代筛选培养、萌发培养、芽诱导培养、生根培养和潮霉素选择培养,获得了完整的抗性再生苗,经PCR和PCR-Southern分析鉴定,初步证明外源PSY基因已整合到大豆的基因组中. Taking somatic embryo of three varieties of soybean as acceptor,PSY gene of soybean was transformed by particle bombardment.Hygromycin resistant regeneration plants of soybean were obtained by somatic embryo propagating cultivation,bourgeoning cultivation,inducting bud and striking root cultivation and hygromycin screening in selective condition.PCR and PCR-Southern analysis from the transferred gene plants proved that PSY has been transferred into genome of soybean.
作者 抗艳红 龚学臣 季静 王萍 王罡 KANGYan-hong~1,GONGXue-chen~1,JIJing~2,WANGPing~3,WANGGang~2(1.Hebei North University,Zhangjiakou,Hebei 075000,China;2.Tianjin university,Tianjin,300072,China;3.Weihai Institute of Technelogy,Marine school,Lianyungang,222005,China)
出处 《河北北方学院学报:自然科学版》 2005年第2期 31-34,共4页 Journa of Hebei North University:Natural Science Edition
基金 国家转基因植物研究和产业化专项基金
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