Objective To study the changes of the ultrastructure of pancreas and distributive pattern of islet with age, to supply reference for the pancreas transplantation. Methods 30 healthy SD rats were divided into 3 groups: young group, adult group and aged group, 10 rats each group. The pancreas heads, body and tail of each rat were removed to make tissue slice for observation with light microscope. Pancreas tails of 3 rats selected from each group were removed for observation with transmission electron microscope. Results TEM showed that in aged rats, the nucleus of their gland cell of the exo-crine portion became pyknotic, the zymogen granules were reduced, the mitochondria were hypertrophic and their cristae fell off, the rough endoplasmic reticula were dilated, the adipose drops appeared and the lysosomes were increased. But, in the endocrine portion, the halo of secretory granules in the B cell were increased in width, reduced in number, the granules of rough surfaced endoplasmic reticulum fell off and the mitochondria were reduced. In A cell no obvious changes occurred with age. The aged rats were observed by LM and the connective tissues between the lobular pancreatises were hyperplastic. The distribution . of pancreas islet was large in number in pancreatic tail of young rat. Conclusion The gland cell of exocrine and the B cell of endocrine of pancreas occurred in the structural change correlated to the age. The distribution of pancreas islet was large in number in pancreatic tail of young rat. It is suggested that in the donor for pancreas transplantation the tail from young rat is preferable to that of the aged.
Sichuan Journal of Anatomy