期刊文献+

一种饵料微藻的脂肪酸甾醇分析及化学分类的探讨 预览 被引量:11

Analysis of fatty acids and sterols in one marine feed microalga and its potential chemotaxonomic significance
在线阅读 下载PDF
收藏 分享 导出
摘要 对一种重要的未知生物饵料微藻在充气和瓶中静态两种生态条件下进行培养,设计了一种分步衍生化脂肪酸与甾醇的GC/MS连续分析法,对微藻脂肪酸甾醇进行了定性和定量分析,结果表明,这种微藻主要含有丰富的二十碳五烯酸(EPA),C14:0,C16:0,C16:1以及一定量的二十二碳六烯酸(DHA),C22:5(n=6)和C18不饱和脂肪酸;共鉴定出11种甾醇组分,主要是24-甲基(乙基)胆甾醇,碳数分布从27到30,特别含有甲基巴夫甾醇和乙基巴夫甾醇;可以把这种微藻归到Prymnesiophyceae纲Pavlovales目Pavlovaceae科.通过实例分析证明,脂类分析结果可以作为海洋微藻化学分类学的一个重要依据. There is an unknown microalga(ZM2020) which has been used widely and proved to be nutritious as feed. In order to evaluate its nutrition value based on fatty acids and sterols, the alga has been cultured in two modes as standing and air bubbling conditions, simultaneous analysis of fatty acids and sterols has been carried out on a gas chromatography mass spectrometer by applying a new step-wise derivetization method. The results indicate that the microalga is abundant in EPA, C14:0, C16:0, C16:1, as well as some conceivable amount of DHA, C22:5(n-6) and C18 unsaturated fatty acids. Eleven C27 to C30 sterols have been identified with major ones including 24-methyl (ethyl)-cholesterol. The existence of unusual sterols of methylpavlovol and ethylpavlovol has been observed unambiguously. Based on the composition analysis of fatty acids and sterols, this alga can be classified into Prymnesiophyceae, Pavlovales, Pavlovaceae. It is also demonstrated here that lipid analysis can be a useful tool for microalgal chemotaxonomy.
作者 徐继林 严小军 朱艺锋 王晓申 俞红 XU Ji-lin~1,YAN Xiao-jun~1,ZHU Yi-feng~1, WANG Xiao-shen~1,YU Hong~1(1.Key Laboratory of Marine Biotechnology, Ningbo University,Ningbo 315211, China)
出处 《海洋学报》 CAS CSCD 北大核心 2005年第4期 121-126,共6页 Acta Oceanologica Sinica
基金 国家新药基金资助项目(2003AA2Z3511) 浙江省青年人才基金资助项目(RC02059) 浙江省教育厅资助项目(20030455).
关键词 脂肪酸 甾醇 海洋饵料微藻 化学分类学 fatty acids sterols marine feed microalgae chemotaxonomy
  • 相关文献

参考文献17

  • 1BLIGH E G,DYER W J.A rapid methodlipid extraction and purification[J].Can J Biochem Physiol,1959,37(5):911-917. 被引量:1
  • 2詹益兴.实用气相色谱分析[M].,1990.186. 被引量:3
  • 3GHOSH P, PATTERSON G W, WIKFORS G H. Sterols in some marine Prymnesiophyceae[J]. J Phycol , 1998, 34(4): 511-514. 被引量:1
  • 4VOLKMAN J K, FARMER C L, BARRETT S M. Unusual dihydroxysterols as chemotaxonomic markers for microalgae from the order Pavlovales(Haptophyceae)[J] . J Phycol,1997, 33(9): 1 016-1 023. 被引量:1
  • 5曾志,向同寿,曹陇梅.甾醇及其衍生物的质谱学规律(I).△^5,22—,△^5,24(25)—,△^5,24(28)—甾...[J].质谱学报,1994,15(2):11-16. 被引量:9
  • 6GLADU P K, PATTERSON G W, WIKFORS G H, et al. Free and combined sterols of Pavlova gyrans[J] . Lipids, 1991, 26(7):656-659. 被引量:1
  • 7LEWIS T E, NICHOLS P D, MCMEEKIN T A. Sterol and squalene content of a docosahexaenoic-acid-producing thraustochytrid:influence of culture age, temperature, and dissolved oxygen[J]. Marine Biotechnology, 2001,3(5): 439-447. 被引量:1
  • 8LEBLOND J D, CHAPMAN P J. A survey of the sterol composition of the marine dinoflagellates Karenia brevis, Karenia mikimotoi, and Karlokinium micrum: distribution of sterols within other members of the class dinophyceae[J]. J Phycol, 2002, 38(5): 670-682. 被引量:1
  • 9WIKFORS G H, GLADU P K, PATTERSON G W. In search of the ideal diet for oysters: recent progress with emphasis on sterols[J].J Shellfish Res, 1991,10(3): 292-301. 被引量:1
  • 10HOLDEN M J, PATTERSON G W. Absence of sterol biosynthesis in oyster tissue culture[J]. Lipids, 1991,26(1) :81-82. 被引量:1

二级参考文献65

共引文献51

同被引文献204

引证文献11

二级引证文献62

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈