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灌注式RCCS生物反应器CFD模拟分析微载体旋转对细胞氧供的影响 预览

Stimulation Analysis of Perfusion Rotary Cell Culture System Bioreactors with Computational Fluid Dynamic Software
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摘要 目的:探讨利用微重力旋转细胞培养系统(RCCS)生物反应器仿真模拟分析微载体旋转对细胞氧供的影响。方法:联立不可压缩牛顿流体连续性方程和动量守恒方程、微载体内以及流动空间中氧的传递和反应方程,设置参数并求解;以Gamb it建立RCCS网格模型,并用F luent软件在欧拉多相模式下进行生物反应器数值模拟及流体力学分析。结果:以微载体直径及RCCS旋转速度作为自变量,基于欧拉-欧拉多相模型和氧运输公式成功建立RCCS数值分析模型。微载体直径和旋转速度均影响细胞培养效率,直径200、300、400μm的微载体旋转速度分别为10、12、14 r/m in;旋转1 h后直径600μm的微载体RCCS中间区平均氧浓度增加85%。结论:CFD可以定量模拟分析影响微载体及氧供的相关因素,为优化培养条件提供参数。 Objective To simulate the rotary cell culture system(RCCS) liver bioreactor model with computational fluid dynamic(CFD) software and to analyze the characteristic of nutrition supply.Methods A numerical analysis of the RWV bioreactor was conducted by incorporating the Eulerian-Eulerian multiphase and oxygen transport equations.The bead type,size and rotating speed were the control variables in the calculations.Results The present results showed that the rotating speed for appropriate suspensions should to be increased as the size of the bead/cell increasing:10 r/min for 200 μm;12 r/min for 300 μm.As the rotating speed and the bead size increasing from 10 r/min(200 μm) to 18 r/min/(600 μm),the mean oxygen concentration in the 80% midzone of the vessel was increased to 85% after 1 hour rotation due to the high convective flow for 18 r/min(600 μm) case as compared to 10 r/min(200 μm) case.Conclusion The present results may serve as criteria to set the operating parameters for a RWV bioreactor,it might provide a design parameter for an advanced suspension bioreactor for 3D engineered cell and tissue culture.
作者 车军 田卫群 周焕城 张志 高毅 刘勇 CHE Jun1,2,TIAN Wei-qun1,ZHOU Huan-cheng4,ZHANG Zhi4,GAO Yi4,LIU Yong3,4(1Basic Medical College,Wuhan University,Wuhan,Hubei 430000;2Department of Emergency,3Department of ICU,Renmin Hospital,Hubei University of Medicine,Shiyan,Hubei 442000;4Institute of Regenerated Medicine,South Medical College,Guangzhou,Guanagdong 510515,China)
出处 《湖北医药学院学报》 CAS 2011年第4期360-363,共4页
基金 国家863高技术发展计划资助(2006AA02A141)
关键词 微重力旋转培养系统(RCCS) 生物反应器 CFD软件 Rotary cell culture system Bioreactor Computational fluid dynamics software
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参考文献14

  • 1刘兴茂,刘红,熊福银,陈昭烈.模拟微重力条件下心肌细胞的体外三维固定化培养[J].中国生物工程杂志,2003,23(5):91-94. 被引量:6
  • 2M hler L,Flockerzi D,Sann H,et al.Mathematicalmodel of influenza A virus production in large-scale mi-crocarrier culture. Biotechnology and Bioengineering . 2005 被引量:1
  • 3Ge L,Ratcliffe M.The use of computational flowmodel-ing(CFD)to determine the effect of left ventricularshape on blood flow in the left ventricle. The Annals of Thoracic Surgery . 2009 被引量:1
  • 4Chen G,Palmer AF.Hemoglobin-based oxygen carrierand convection enhanced oxygen transport in a hollowfiber bioreactor. Biotechnology and Bioengineering . 2009 被引量:1
  • 5Sullivan JP,Palmer AF.Targeted oxygen delivery withinhepatic hollow fiber bioreactors via supplementation ofhemoglobin-based oxygen carriers. Biotechnology Progress . 2006 被引量:1
  • 6Als-Nielsen B,Gluud LL,Gluud C.Nonabsorbable disaccharides for hepatic encephalopathy. Cochrane Database of Systematic Reviews . 2004 被引量:1
  • 7POLLACK S R,MEANEY D F,LEVI NE E M,et al.Nu-merical model and experi mental validation of microcarrier mo-tionin a rotating bioreactor. Tissue Engineering . 2000 被引量:1
  • 8Wang Y,Yan L.CFD Studies on Biomass Thermochemical Conversion. Int J Mol Sci . 2008 被引量:1
  • 9Shi LI,Zhaofeng MA,Zhongying NIU,et al.NASA-approvedrotary bioreactor enhances proliferation and osteogenesis of hu-man periodontal ligament stem cells. Stem Cells and Development . 2009 被引量:1
  • 10Consolo F,Fiore GB,Truscello S,et al.A computational model for the optimization of transport phenomena in a rotating hollow-fiber bioreactor for artificial liver. Tissue Eng Part C Methods . 2009 被引量:1

二级参考文献8

  • 1Langer R S, Vaeanti T P. Tissue engineering. Science, 1993,260:920 ~ 925. 被引量:1
  • 2Li R K, Yau T M, Weisel R D, et al. Construction of bioengineering cardiac graft.J Torac Cardiovasc Surg,2000,119(2) :366 ~ 375. 被引量:1
  • 3Molnar G, Schroedl N A, Gonda S, et al. Skeletal muscle satellite cells cultured in simulated microgravity. In Vitro Cell Dev. Biol.Anim. 1997,33(5) :386 ~ 342. 被引量:1
  • 4Goodwin T J,Prewett T L,Wolf D A,et al. Reduced shear stress:a major component in the ability of mammalian tissues to form three-dimensional assemblies in simulated microgravity. J Cell Biochem,1993,51(4) :301 ~ 311. 被引量:1
  • 5Freed L E, Vunjak-Novakovic G, Langer R. Cultivation of cell-polymer cartilage implants in bioreaetors.J Cell. Biochem, 1993,51(6) :257 ~ 263. 被引量:1
  • 6Harding S E, Davies C H, Wynne D G, et al. Contractile function and response to agonists in myocytes from failing human heart. Eur Heart J,1994,15(3) :35 ~ 41. 被引量:1
  • 7Freed L E, Vunjak-Novakovic G. Microgravity tissue engineering. In Vitro Cell Dev Biol, 1997,33(5) :381 ~ 385. 被引量:1
  • 8Akins R E, Boyce R A, Madonna M L, et al. Cardiac organogenesis in vitro: Reestablishment of three-dimensional tissue architecture and function by dissociated ventricular cells. Tissue Engineering,1999,5(2) : 103 ~ 109. 被引量:1

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