Objective： To investigate whether immature rabbit hearts could be protected after safflor yellow （SY） preconditioning. Methods： twenty-one healthy white rabbits were randomly divided into 3 groups, control group, ischemic preconditioning （IPC） group, safflor yellow injection （20mg/ml） group. In IPC group, the hearts were first subjected to an IPC stimulus consisting of 5 minutes of global ischemia plus 10 minutes of reperfusion, In safflor yellow group, the hearts were perfused by safflor yellow （20mg/ml） 20 minutes. The hearts in 3 groups were subjected to 30 minutes of global ischemia and 60 minutes of reperfusion. Krebs-Henseleit buffer bubbled with a 95%O2 ＋ 5% CO2 gas mixture perfused the hearts in langendorff model at 10mmHg. During and after reperfusion, the change of myocardial hemodynamics, myocardial enzyrnein and the coronary effluent and iNOS were determined. Results： LVDP（ ± dp/dtmax）, CF recovery had a significant improvement in safflor yellow group. LDH, CK levels were the least in safflor yellow group, and safflor yellow group also had a higher level of tissue iNOS. Conclusions： Safflor yellow preconditioning had protective effects on rabbit isolated hearts.
Pharmacology and Clinics of Chinese Materia Medica