目的制备3种具有良好稳定性的胶原-壳聚糖多孔支架, 初步探讨其植入体内后的组织相容性. 方法冻干法制备胶原-壳聚糖多孔支架,分别采用真空干热和戊二醛对其交联,制备未交联(材料1)、真空干热交联(材料2)和戊二醛交联(材料3)3种胶原-壳聚糖多孔支架,扫描电镜观察支架微观结构.将12只家兔随机分为4组(n=3),3种支架材料分别交叉埋植入12只家兔耳背部皮下,术后观察、记录全身情况及手术区变化,分别于术后3、7、14及28 d组织切片下见3种材料的稳定性和组织相容性. 结果扫描电镜下见3种支架均具有三维多孔结构,孔径分别为120±10、80±15和170±20 μm,孔隙率均大于90%.大体观察:实验家兔术后全身情况良好,手术区红肿轻微,切口均愈合良好.组织学观察:材料1降解吸收迅速,能引起明显的炎性反应,材料2降解较快,炎性反应不及材料1明显,材料3降解较慢,炎性反应轻微,术后组织再生较快. 结论胶原-壳聚糖多孔支架具有适合组织再生的三维多孔结构,交联能提高胶原-壳聚糖多孔支架的早期稳定性和组织相容性,戊二醛交联优于真空干热交联.
Objective To construct three kinds of collagen chitosan porous scaffolds with enhanced biostability and to investigate the histocompatibility of the scaffolds in vivo. Methods Collagen-chitosan porous scaffolds were fabricated by freeze-drying method, cross-linked using dehydrothermal treatment and glutaraldehde, respectively. The morphology of the uncross-linked scaffold （scaffold 1 ）, dehydrothermally cross-linked scaffold （scaffold 2） and glutaraldehde cross linked scaffold （scaffold 3） was studied by scanning electron microscopy. Three kinds of scaffolds were embedded subcutaneously on dorsal surface of 12 rabbit ears. The general and local responses were recorded daily. The biostability and histocompatibility of the scaffolds were observed by using HE staining after 3, 7, 14 and 28 days of operation. Results The scaffolds had three dimensional porous structures with a porosity of more than 90%, and possessed pore sizes of 120±10 μm, 80±15 μm and 170±20 μm, respectively. All experimental rabbits survived with good general condition during the study. All skin incisions healed well without obvious reactive red or swelling. Histological study showed that scaffold 1 was degraded rapidly with obvious inflammation. The degradation of scaffold 2 was slower than that of scaffold 1 and the inflammation of scaffold 2 was also milder than that of scaffold 1. Scaffold 3 possessed slow degradation property with slight inflammatory reaction, and rapid tissue regeneration. Conclusion The collagen-chitosan porous scaffolds have three-dimensional porous structures that are suitable for tissue regeneration. The biostability and histocompatibility of the scaffolds are enhanced after cross-linked. Glutaraldehde cross-linked is better than dehydrothermally cross-linked, which can facilitate dermal tissue reconstruction.
Chinese Journal of Reparative and Reconstructive Surgery