Objective To compare antibacterial activity of antibacterial protein in the Musca dornestica larvae by five inducements and observe the effect of the different inducements. Methods The M. dornestica larvae were treated by S. aureus,E, coli, LPS, ultraviolet radistion and heat-shock. Antibacterial protein by five inducements were separated at different time,detected their antibacterial activity to observe the peak time of antibacterial activity by five inducements, and compared antibacterial activity at the peak of antibacterial protein by five inducements. Results The peak of antibacterial activity appeared at 36-48 h after induced by four inducements （Because many M. dornestica larvae pupated,we did not observe the peak of antibacterial activity by heat-shock）. The antibacterial activity of antibacterial proteins by S. aureus was best,and the antibacterial activity of antibacterial proteins by heat-shock was better than control group. Conclusion The peak of antibacterial proteins by different inducements appeared at different time. The antibacterial activity of antibacterial proteins by injected bacteria was best, and heat-shock was also a better method.
Chinese Journal of Vector Biology and Control
Musca domestica larva