期刊文献+

不同诱导源诱导家蝇幼虫抗菌蛋白效果的比较 预览 被引量:5

Comparison of the Effect of Different Inducements Inducing the Antibacterial Proteins in the Musca domestica Larvae
在线阅读 下载PDF
收藏 分享 导出
摘要 目的比较5种诱导源所诱导产生家蝇幼虫抗菌蛋白的抗菌活性,观察不同诱导源对家蝇幼虫抗菌蛋白产生的诱导效果.方法用金黄色葡萄球菌、大肠埃希菌、大肠埃希菌脂多糖、紫外线及热诱导家蝇幼虫,在诱导后不同时间提取抗菌蛋白进行抑菌实验,观察不同诱导源诱导后抗菌蛋白产生的高峰时间,并比较各诱导组高峰时间产生抗菌蛋白的抗菌活性.结果各诱导组诱导家蝇幼虫产生抗菌蛋白达最大活性时间为诱导后36~48 h(热诱导后家蝇幼虫很快化蛹,未观察抗菌蛋白产生高峰时间);金黄色葡萄球菌诱导后的抗菌蛋白抗菌活性最佳,热诱导后的抗菌蛋白抗菌活性也较未诱导组好.结论各诱导源诱导后抗菌蛋白产生的高峰时间不同;细菌诱导的抗菌蛋白抑菌效果最好,热诱导也是一种较理想的诱导方法. Objective To compare antibacterial activity of antibacterial protein in the Musca dornestica larvae by five inducements and observe the effect of the different inducements. Methods The M. dornestica larvae were treated by S. aureus,E, coli, LPS, ultraviolet radistion and heat-shock. Antibacterial protein by five inducements were separated at different time,detected their antibacterial activity to observe the peak time of antibacterial activity by five inducements, and compared antibacterial activity at the peak of antibacterial protein by five inducements. Results The peak of antibacterial activity appeared at 36-48 h after induced by four inducements (Because many M. dornestica larvae pupated,we did not observe the peak of antibacterial activity by heat-shock). The antibacterial activity of antibacterial proteins by S. aureus was best,and the antibacterial activity of antibacterial proteins by heat-shock was better than control group. Conclusion The peak of antibacterial proteins by different inducements appeared at different time. The antibacterial activity of antibacterial proteins by injected bacteria was best, and heat-shock was also a better method.
作者 刘晖 万启惠 贺莉芳 张曦 LIU Hui, WAN Qi-hui, HE Li-fang, et al. (Zunyi Medical College, Zunyi 563003,China)
出处 《中国媒介生物学及控制杂志》 CAS CSCD 北大核心 2005年第6期 418-420,共3页 Chinese Journal of Vector Biology and Control
基金 贵州省科技厅科研项目
关键词 家蝇幼虫 诱导 抗菌蛋白 抑菌活性 Musca domestica larva Inducement Antibacterial protein Antibacterial activity
  • 相关文献

参考文献6

  • 1吴赠 赵慰先.人体寄生虫学[M](第3版)[M].北京:人民卫生出版社,1983.1055-1056. 被引量:2
  • 2张颖悟主编..临床微生物学[M].大连:大连出版社,1990:456.
  • 3刘晖,万启惠,张曦,贺利芳,黄学贵.诱导家蝇幼虫抗菌蛋白的提取及动力学研究[J].中国公共卫生,2004,20(4):427-428. 被引量:8
  • 4叶应妩 王毓三.全国临床检验操作规程[S].第2版[Z].中华人民共和国卫生部医政司,1997.556-561. 被引量:1
  • 5Gune H, Heller M, Steiner H. Structure of preproattacin and its processing in ins cells infected with a recombinant baculovirus[J]. Eur J Biochem, 1990,187: 699-703. 被引量:1
  • 6钟文彪 黄自然 卢蕴良.2’,5’—寡腺苷酸诱导家蚕对细胞多角体病毒抑制作用的研究[J].科学通报,1982,12:761-763. 被引量:1

二级参考文献6

共引文献7

同被引文献70

引证文献5

二级引证文献26

投稿分析
职称考试

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈