目的 观察家蝇幼虫抗菌蛋白对黑色素瘤A375细胞凋亡的诱导，探讨其抗肿瘤作用机制。方法 流式细胞仪测定家蝇幼虫抗菌蛋白对细胞周期和细胞凋亡的影响；HE染色法观察细胞凋亡形态，并测定细胞凋亡指数（AI）。结果 在0．10％组A375的G／M期升高，S期降低。0．50％组G0／G1期、G2／M期和AI／PI（增殖指数）显著增加，S期及PI明显减少。2．50％组S期、PI、AI／PI及细胞凋亡率（Ap）显著增高，G0／G1期大大减少。12．50％组G2／M期、PI、AI／PI及Ap显著增高，G0／G1期和S期明显下降。结论 家蝇幼虫抗菌蛋白对黑色素瘤A375细胞有明显的抑杀作用；家蝇幼虫抗菌物质可能系通过细胞周期阻滞以及诱导细胞凋亡而抑制黑色素瘤A375细胞生长。
Objective To investigate the inducement of the antibactarial materal from the Musca domestica larvae to the A375 apoptosis to explore it is antitumor mechanism. Methods The effects of the antibactarial materal from the M. domestica larvae on the cell cycle and apoptosis of A375 were measured by flow cytometry. The morphology of apoptosis cells was observed with the hematoxylin-eosin stainings, and the apoptosis index（AI） was measured. Results Of the A375 cell cycle, percentage of G2/M was increased, and the percentage of S was decreased in the group treated by 0.10% the antibactarial material. The percentage of G0/G1 , G2/M and AI/PI were much increased, both the percentage of S and PI were much decreased in the 0.50% group. In the 2.50 % groups, the percentage of S, PI, AI/PI and apoptosis were significantly increased, the percentage of G0/G1 was much decreased. At the 12.50% group, the percentage of G2/M, PI, AI/PI and apoptosis were significantly increased, the percentage of G0/G1 and S were much decreased. Conclusion The antibactarial materal of the M. domestica larvae could inhibited and killed the A375 cell. The growth of A375 may be inhibited by the antibactarial materal blocking cell cycle and inducing the apoptosis of cell.
Chinese Journal of Vector Biology and Control
Musca domestica larva