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苹果茎沟病毒梨分离物外壳蛋白基因的克隆和序列分析 预览 被引量:8

Cloning and sequence analysis for the CP gene of Apple stem grooving virus from pears
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摘要 选取生物学和血清学特性存在较大差异的苹果茎沟病毒(Apple stem grooving virus,ASGV)梨分离物P-6-1-17、P-4-1-69、P-L2和P-D-21,采用RT-PCR法对3′端进行扩增,所获扩增片段经序列测定,全长分别为1089nt(P-6-1-17、P-L2)和1069nt(P-4-1-69、P-D-21)。4个分离物的CP基因均由714nt组成,其核苷酸和推导编码蛋白氨基酸序列同源性分别为89.8%~96.4%和94.9%~97.9%。将我国4个分离物的CP基因与已报道ASGV分离物及分子变种进行序列同源性比较和系统进化树分析,结果表明我国ASGV分离物至少可分为2个组群,其中生物学表现明显不同于其它分离物的P-L2,其CP基因核苷酸序列显示较大的变异。 Four isolates of Apple stem grooving virus, P-6-1-17, P4-1-69, P-L2 and P-D-21 from pears in China, which displayed different reactions in biological and serological tests, were studied for their molecular characteristics. RT-PCR products of 3′end of the four isolates were cloned and sequenced. The sizes of cloned fragments are 1089 nt from P-6-1-17 and P-L2, and 1069 nt from P-4-1-69 and P-D-21, respectively, which include the 714 nt CP-coding region. The similarities of nucleotide and deduced amino acid sequences of the CP genes among these isolates are 89.8% to 96.4% and 94.9% to 97.9%, respectively. The sequence data obtained in the study were compared with previously reported sequences of ASGV isolates and sequence variant species from different sources. Results suggested that isolates P-6-1-17, P-4-1-69,P-L2 and P-D-21 should be divided into two different subgroups. P-L2 showed different reactions in biological test and its CP gene also has a relatively high variability.
作者 郑银英 洪霓 王国平 胡红菊 ZHENG Yin-ying , HONG Hi, WANG Guo-ping, HU Hong-ju (1.College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. National Indoor Conservation Center of Virus-free Gemplasms of Fruit Crops,Wuhan 430070, China; 3.Research Institute of Fruit, Tea and Silkworm-Mulberry, Hubei Academy of Agricultural Sciences ,Wuhan 430209 ,China)
出处 《植物病理学报》 CAS CSCD 北大核心 2006年第1期 62-67,共6页 Acta Phytopathologica Sinica
基金 国家自然科学基金资助项目(30370997) 科技部“八六三”资助项目(2001AA241142)
关键词 苹果茎沟病毒 梨分离物 CP基因 序列分析 变异 ASGV pear isolates CP gene sequence analysis variability
作者简介 郑银英(1975-),女,吉林龙井市人,博士,研究方向为植物病毒学。 通讯作者:洪霓,教授,主要从事植物病毒学研究;E-mail:whni@mail.hzau.edu.cn
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参考文献13

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