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山茶属红山茶组植物的RAPD分析及分类研究 预览 被引量:22

RAPD Analysis and Taxonomy of Sect. Camellia Species in Camellia
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摘要 以山茶属29种红山茶组植物叶片为材料抽提DNA,利用筛选出的23种随机引物对其进行PCR扩增,将扩增出的谱带转化成0-1型数据,用UPGMA法进行相关分析和聚类分析.结果表明:可以将山茶属红山茶组的29个种划分为4大类,第1类为光果红山茶亚组,第2类为毛蕊系,第3类为滇山茶系,第2类和第3类属于滇山茶亚组,短管红山茶单独聚为一类.其结果与张宏达分类系统基本一致.根据各种之间的相容关系系数的大小,发现红山茶组内有3组亲缘关系较为相近的种. The leaf DNA samples of 29 species of Sect. Camellia in Camellia were extracted and analyzed, using random amplified polymorphic DNA (RAPD) genetic markers. 23 selected primers were used for DNA amplification. The amplified polymorphic DNA bands were translated into 0 - 1 data, and then, correlation analysis and clustering analysis were done for processing data by using UPGMA method. The results indicated that 29 species of Section Camellia should be grouped under four clusters, that is, Subsect. Lucidissima, Series Chrysanthae and Series Reticulata, moreover, C. glabsipelata was taken as a sole cluster. Series Chrysanthae and Series Reticulata composed Subsect. Reticulata. The results were in agreement with the views of Zhang Hongda' s taxonomy. Based on similarily coefficient between each other, 3 varieties groups with close genetic relationship were discovered in Section Camellia.
作者 邓白罗 谭晓风 漆龙霖 贺晶 胡芳名 Deng Bailuo, Tan Xiaofeng, Qi Longlin, He Jin, Hu Fangming ( 1. College of Resources and Environment, Central South University of Forestry & Technology Changsha 410004;2. The Key Laboratory of Non- Wood Forest Product of State Forestry Administration, Ceatrat South University of Forestry & Technology Changsha 410004;3. The Editorial Department of Journal of Central South Forestry University Changsha 410004)
出处 《林业科学》 CAS CSCD 北大核心 2006年第5期 36-41,共6页 Scientia Silvae Sinicae
基金 国家自然科学基金"山茶属植物分子分类与鉴别"项目(39570603).
关键词 山茶属 红山茶组 分类系统 RAPD分析 Camellia Section Camellia taxonomy RAPD analysis
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