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MTT法和LDH法检测T—AK细胞活性的研究 预览 被引量:13

Study on Determination of Cytotoxicity of T-AK Cells with MTT Colorimetry and LDH Release Assays
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摘要 采用MTT比色分析法和LDH释放法检测了CD3单克隆抗体和白细胞介素-2共同激活的T-AK细胞的杀伤活生,并对检测方法进行了改良。 The cytotoxicity of anti-CD3 monoclonal antibody and interleukin-2 costimulated killer cells-T-AK (T-activated killer)cells was detected with MTT colorimetric assay and Lactic dehydrogenase(LDH)release assay,and the determinating assays were improved in the study. The results showed that T-AK cells had potent cytolytic activity on human leukemia K562 cells. After our improvement, MTT assay and LDH assay became simple,convenient,stable and trustworthy in the determination of cytotoxicity. MTT colorimetric assay was much more sensitive than LDH release assay in determinating the cytolytic activity of tumor killer cells.
作者 魏虎来 Wei Hulai (Institute of Hematology,Lanzhou Medical College)
机构地区 血液病研究所
出处 《兰州医学院学报》 1996年第3期 9-11,共3页 Journal of Lanzhou Medical College
基金 甘肃省中青年科技基金项目(ZQ—93—14)
关键词 T-AK细胞 MTT比色法 LDH释放法 细胞毒 活性 T-activated killer(T-AK)cells K562 cells MTT colorimetry LDH release cytotoxicity
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