目的 调查2005年1～8月分离自江西宜春金黄色葡萄球菌的耐药情况。方法 采用常规方法分离金黄色葡萄球菌，用全自动微生物分析仪进行菌种鉴定和药敏试验，采用苯唑西林纸片扩散法检测耐甲氧西林金黄色葡萄球菌（MRSA）。结果 金黄色葡萄球菌主要分离自痰液和尿液，MRSA的分离率为62．1％；MRSA对氨苄西林／舒巴坦、青霉素和头孢唑林的耐药率均为100％，而MSSA均为0；所有的分离株对呋喃妥因和万古霉素都敏感；MRSA对克林霉素、红霉素、庆大霉素、四环素、利福平、复方新诺明和左氧氟沙星的耐药率分别为70．7％、86．6％、87．8％、82．9％、42．7％、30．5％和91．5％，而MSSA分别为32．0％、42．0％、20．0％、20．0％、10．0％、30．0％和6．0％。结论 MRSA的耐药性较为严重，而MSSA除青霉素外耐药率较低，万古霉素对MRSA的体外抗菌活性非常强。
OBJECTIVE To investigate the antimicrobial-resistant profile of Staphylococcus aureus isolated from clinical specimens. METHODS The isolates of S. aureus were obtained from all kinds of clinical specimens by routine methods. The identification and susceptibility test of the isolates were determined by Automated Microbiology Analyzer. Disk-diffusion was used for detecting meticillin-resistant Staphylococcus aureus（MRSA）. RESULTS The isolates were mainly isolated from sputa and urines. 62.1% of isolates were referred as MRSA. All isolates of MRSA were resistant to ampicillin/sulbactam, penicillin G and cefazolin. No isolates of MSSA were resistant to above drugs. All isolates were susceptible to nitrofurantoin and vancomycin. The resistant rates of the isolates of MRSA to clindamycin, erythromycin, gentamicin, tetracycline, rifampin, sulfamethoxazole and levofloxacin were 70.7%, 86.6%, 87.8%, 82.9%,42.7%, 30.5% and 91.5%, respectively, those of MSSA were 31.3%, 41.7% ,20.8% ,20. 8% ,10.4% ,29.2% and 6.3%. CONCLUSIONS The antimicrobial resistance of MRSA is serious, but the antibiotics have high activity to the isolates of MSSA except penicillin G in vitro. Vancomycin has very high activity to the isolates of MRSA in vitro.
Chinese Journal of Nosocomiology