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苦丁茶冬青叶多糖的提取及含量测定 预览 被引量:13

Extraction and Determination of the Polysaccharides from the Leaves of Ilex kudincha C. J. Tseng
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摘要 目的 研究苦丁茶冬青叶多糖含量的测定方法。方法 以水提醇沉的方法从苦丁茶冬青叶中提取得到苦丁茶冬青叶多糖并以正交实验优化显色条件,采用苯酚-硫酸法测定其多糖含量。结果 测定波长为482nm,最佳显色条件为6%苯酚用量1.0ml,浓硫酸用量7.0ml,反应时间40min。标准曲线回归方程为A=0.0546C-0.0033(n=10),r=0.9998,并在2.51-45.11mg·L^-1范围内呈良好线性关系。平均回收率为98.55%,RSD=1.86%(n=5)。测得多糖平均含量为31.90%,RSD=1.75%(n=6)。结论 该方法简单、准确、重现性好,可为其继续研究开发及应用提供参考依据。 Objective To study the determination method for polysaccharides in the leaves of llex kudincha C. J. Tseng. Methods The leaves of llex kudincha C. J. Tseng were defatted with 95% EtOH, extracted with water and precipitated with 80% EtOH, and then was deproteined three times by Scrag method, and from which, a crude polysaccharide noted as KPSⅡ was obtained. The traditional colofimetric method applied for the determination of polysaccharides from the leaves of Ilex kudincha C. J. Tseng was developed by the use of phenylhydrate - sulfuric acid, and was optimized by orthogonal experiment. Results The results showed that the wavelength for measurement was 482 nm. The best colorimetric conditions were the time of reaction of 40 rain and the dosage of 6% phenylhydrate and H2SO4 of 1.0 and 7.0 ml, respectively. Linear range was 2.51 - 45.11 mg·L^-1 (A =0.054 6C -0. 003 3 ,r =0.999 8 ). The average recovery was 98.55% and RSD was 1.86% ( n = 5 ). The pelysaccharide content of KPS Ⅱ was 31.90% and RSD was 1.75% (n =6). Conclusion The method used in this paper is simple, accurate and stable. The result obtained may act as a base for the further study and application of it.
作者 王新 陆慧宁 林少琨 WANG Xin, LU Hui-ning, LIN Shao-kun( 1. Instrumental Analysis & Research Center, Sun Yat - sen University, Guangzhou 510275, China; 2. Depart- ment of Pharmaceutical Engineering, Liaoning University, Shenyang 110036, China)
出处 《时珍国医国药》 CAS CSCD 北大核心 2006年第10期 1977-1979,共3页 Lishizhen Medicine and Materia Medica Research
关键词 苦丁茶冬青叶多糖 提取 苯酚-硫酸法 含量测定 正交实验 Polysaccharides from the leaves of Ilex kudincha C. J. Tseng Extraction Phenylhydrate -sulfuric acid method Determination OrthogonaL experiment
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  • 1朱莉芬,李美珠,钟伟新,李爱华,罗集鹏,房志坚.苦丁茶的心血管药理作用研究[J].中药材,1994,17(3):37-40. 被引量:48
  • 2吉林农业大学长白山资源开发研究所.人参科研论文集[M].,1998.230. 被引量:1
  • 3李晓辉.-[J].中草药,1997,28(9):530-530. 被引量:1
  • 4Dong Q, Zheng L Y, Fang J N. Study on the determination of oligosaccharide and polysaccharide by modified phenol-sulfuric acid method [J]. China J Chin Mater Med (中国中药杂志),1996, 31(9); 550. 被引量:1
  • 5Liu H, Xue H Z, Yang W G, et al. Extraction and determination of polysaccharide from Zea mays [J]. J Nanjing Univ Tradit Chin Med (南京中医药大学学报), 1999, 15(2): 90. 被引量:1
  • 6Wang R C, Chen Z C, Sun C J. Monograph on Biological Organic Chemistry (生物有机化学专论) [M]. Jinan: Shandong Science and Technology Press, 1990. 被引量:1
  • 7司世麟,生物化学与生物物理进展,1986年,5卷,68页 被引量:1
  • 8Xiang D-B, Li X-Y. Antitumoractivity and immuno-potentiating actions of Achyranthes bidentata polysaccharides. ActaPharmacol Sin (中国药理学报), 1993,14:556-561. 被引量:1
  • 9Zhang Z-Y (张志英), Wang S-K (王绍坤), Guo L-K (郭连魁). Aging changes ofneurotensin-like and dynorphin-like neurons in the central nucleus of amygdala of the ratanti-aging effect of radix achyranthis bidentatae-immunohistochemical and imagequantitative analytical study. Chin J Anat (解剖学杂志), 1996, 19:123-127. (inChinese with English abstract) 被引量:1
  • 10Li Z-K (李宗锴), Li D-D (李电东). The immunomodulatory effect of Achyranthesbidentata polysaccharides. Acta Pharm Sin (药学学报), 1997, 32:881-887. (in Chinesewith English abstract) 被引量:1

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