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烟草中游离植物甾醇的GC/MS/SIM分析 预览 被引量:9

Determination of Free Phytosterols in Tobacco by Gas Chromatography/Mass Spectroscopy/ Selected Ion Monitoring
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摘要 建立了一种采用二氯甲烷提取、微孔滤膜过滤、气相色谱/质谱/选择离子监测(GC/MS/SIM)定量测定烟草中游离植物甾醇的方法,并采用该法测定了部分烟叶样品、霉变烟叶和部分卷烟样品中的游离植物甾醇含量。结果表明:①该法的检测限为5.12~8.43ng/g,回收率在93%~103%之间,RSD〈6%;②香料烟的麦角甾醇含量最高,其次是烤烟,白肋烟最低;③卷烟烟丝中的游离植物甾醇含量与其烟气中苯并[a]芘的释放量呈显著正相关。结论:该法适合批量烟叶样品中甾醇的定量分析。 A method for determination of free phytosterols in tobacco was developed with dichloromethane extraction, micropore membrane filtration and gas chromatography/mass spectroscopy/selected ion monitor. The free phytosterols in tobacco samples, mouldy tobacco, and cigarette samples were tested, The results showed that: 1 ) the recovery of free phytosterols was from 93% to 103%, the detection limit was 5,12 to 8.43ng/g and RSD below 6%; 2)the oriental tobacco samples had the highest ergosterol level, followed by Virginia tobacco, and burley tobacco the lowest; 3) the free phytosterols in cigarette filler significantly correlated with the benzo[a]pyrene delivery in mainstream cigarette smoke, The conclusion was that the method was suitable to quantitative analysis of free phytosterols in batches of tobacco samples.
作者 黄龙 王进元 罗诚浩 宋旭艳 马舒翼 柯炜昌 HUANG LONG, WANG JIN-YUAN, LUO CHENG-HAO, SONG XU-YAN, MA SHU-YI, and KE WEI- CHANG (Technology Center of Wuhan Tobacco (Group) Co. , Ltd., Wuhan 430051 , China)
出处 《烟草科技》 EI CAS 北大核心 2006年第10期 41-45,共5页 Tobacco Science & Technology
关键词 气相色谱/质谱/选择离子监测 烟草 植物甾醇 霉变 苯并[A]芘 Gas chromatography/mass spectroscopy/selected ion monitoring Tobacco Phytosterols Moulding Benzo[a] pyrene
作者简介 黄龙(1973-),硕士,武汉烟草集团技术中心工程师,主要从事烟草化学研究。E-mail:hl-0322@yahoo,com.cn.电话:027-68848287
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