The research by man-made cultivation of natural Musca domestica showed leftover meat （bone and skin shrimp） seduced the spawning of natural Musca domestrica maximal extent, and the optimum proportion of brownsuger and powdered milk was 3：1. The optimum moisture of feed which adapted to the growing of Musca domestrica larvae was 80%, and the optimum culture in which the average weight increased of single natural Musca domestrica larvae was max was mixatre which contained bone and wheat bran. Two antibacterial peptides that named MDL-1 and MDL-2 were isolated from larvae hemolymph by means of electrophoretic preparation. The molecule weight of antibacterial peptide 1 （MDL-1） was 10772Da, and MDL-2 was 6091Da, and antibacterial peptides had stronger inhibition of fungi, such as E.coli, S.aureus and B.cereus. MDL-1 had the highest activity against B.cereus, higher activity against E.coli, activity against S.aureus. MDL-2 had the strongest potential activity against E.coli, had stronger potential activity against S.aureus, against B.cereus weakly.