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糖尿病树鼩肝脏病理分析 预览 被引量:2

Pathologic analysis of the liver of experimental diabetic tree shrews
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摘要 目的 对注射链脲佐菌素(STZ)8周后糖尿病树鼩动物肝脏进行病理分析。方法 将树鼩随机分为正常对照组和STZ处理组;静脉注射STZ,共持续8周给药,根据血糖水平,再分为糖尿病组(血糖≥11.1mmol/L)和STZ对照组(血糖〈11.1mmol/L)。分别于成模前和成模后4、8周测定3组动物的血清ALT、AST、IV—C、LN和HA。8周末行肝组织活检,经HE、PAS、D—PAS及Masson染色后光镜下观察。用病理图像分析系统对肝脏胶原纤维面积密度进行分析。以透射电镜观察肝超微结构。结果 注射STZ8周后糖尿病模型动物出现早期肝纤维化的电镜及光镜病理组织学变化。结论 糖尿病树口动物模型注射STZ8周后可出现早期肝纤维化病理改变。 [Objective] To analyse pathologic changes in the liver of experimental diabetic tree shrews. [Methods] Tree shrews were divided randomly into normal control group and Streptozocin (STZ)-treated group. ST-Z-treated animals were then divided into diabetic group and STZ control group. ALT, AST, Type IV collagen (IV-C), Laminin (LN) and Hyaluronic acid (HA) were detected respectively before and after modelling. Liver biopsy was performed at the end of the experiment (8 weeks). All of the liver specimens were observed under light microscope and transmission electron microscope. Collagen area density were detected by computer image analysis system. [Result] Early fibrosis of the liver in diabetic tree shrews can be found by light and electron microscope. [Conclusion] Early fibrosis of the liver in diabetic tree shrews can be found after STZ injection 8 weeks.
作者 朱春晖 冼苏 韦敏怡 秦映芬 周嘉 ZHU Chun-hui, XIAN Su, WEI Min-yi, QIN Ying-fen, ZHOU Jia (1.Department of Andrology, 2.Department of Endocrinology, 3.Department of Pathology, the First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R.China)
出处 《中国现代医学杂志》 CAS CSCD 北大核心 2007年第5期 543-546,共4页 China Journal of Modern Medicine
基金 广西壮族自治区自然科学基金资助项目(桂科基0236020)
关键词 糖尿病 树鼩 肝纤维化 病理改变 diabetes tree shrew liver fibrosis pathologic changes
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