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口虾蛄抗鼻咽癌活性成分的筛选及其对细胞周期的影响 预览 被引量:4

Screening of anticancer ingredient from oratosquilla on nasopharyngeal carcinoma and its effects on cell - cycle
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摘要 目的筛选口虾蛄乙醇提取物抗鼻咽癌活性成分,并探讨其对细胞周期的影响。方法采用系统溶剂法,对口虾蛄乙醇提取物进行分离,以MTT法筛选出对人低分化鼻咽癌CNE-2Z细胞作用最明显的活性成分,然后应用流式细胞术进一步检测其对细胞周期的影响。结果3种提取物对CNE-2Z细胞均有不同程度的抑制作用,且呈明显的剂量依赖性,作用48小时后的乙酸乙酯提取物IC50(239μg/ml)明显低于其余2种(正丁醇提取物IC50:833μg/ml,石油醚提取物:1550μg/ml);流式细胞术检测结果显示,随着乙酸乙酯提取物浓度的增加,G1期和G2/M期细胞所占的百分比逐渐降低,S期细胞所占百分比逐渐增加(P〈0.01)。结论口虾蛄抗鼻咽癌活性成分主要存在于乙酸乙酯提取物中,其作用机制可能与引起细胞周期阻滞于S期有关。 Objective To select the anticancer ingredient from oratosquilla on Cell Line CNE - 2Z of the Nasopharyngeal Carcinoma and study its effects on cell - cycle. Methods The means of systematic solvent extraction was used to separate the 95% ethanol extract from oratosquilla. By using MTT assay. The best effective on the cell growth inhibition of the nasopharyngeal carcinoma cell Line CNE - 2Z were selected in this study. The cell cycle distribution was analyzed by flow cytometry assay. Results The results of MTT assay indicated that the three extracts had different inhibition on the CNE -2Z cells and there was significant correlation between dose and effect as well as between time and effect. And those IC50 of 48h was separately 1550μg/ml, 239μg/ml and 833μg/ml. So the ethylacetate extract was the most effective on the growth inhibition of CNE - 2Z ( P 〈 0.01 ). The proportion of G1 phase and G2/M phase decreased and S phase increased gradually following the concentration of the ethylacetate extract increasing ( P 〈 0.01 ). Conclusion The ethylacetate extract is the most effective on the growth inhibition of CNE - 2Z and it inhibits the cell growth of CNE - 2Z probably by inducing the S phase arrest.
作者 孔霞 黄培春 李明勇 陈锦 KONG Xia ,HUANG Pei - chnu,LI Ming - yong ,et al. ( Guangdong Med. Coll. , Zhanjiang 524023, China)
机构地区 广东医学院
出处 《宁夏医学杂志》 CAS 2007年第7期 584-585,共2页 Ningxia Medical Journal
关键词 口虾蛄 鼻咽癌 筛选 细胞周期 Oratosquilla Nasopharyngeal carcinoma Select Cell cycle
作者简介 孔霞,女(1979-),山东籍,硕士学位,助教,从事鼻咽癌防治研究。
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