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毛细管气相色谱法测定烟草中的甾醇类化合物 预览 被引量:6

Determination of Sterols in Tobacco with Capillary Gas Chromatography
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摘要 以丙酮为提取剂、1%硫酸的乙醇溶液和2.0 mol/L氢氧化钾的乙醇溶液分别作水解剂和皂化剂、三甲基氯硅烷和六甲基二硅胺烷作衍生化剂,采用毛细管气相色谱法测定了15种烤烟烟叶和10种商品卷烟中的胆甾醇、菜油甾醇、豆甾醇和β-谷甾醇含量.结果表明:①胆甾醇、菜油甾醇、豆甾醇和β-谷甾醇的RSD<5%,回收率在94.24%~101.22%;②在所测定的烟草样品中,不管是烤烟烟叶,还是烤烟型或混合型卷烟,均是豆甾醇和β-谷甾醇的含量较高,菜油甾醇的含量次之,胆甾醇的含量最低;③同一产地不同部位烟叶样品总甾醇的含量为下部烟>中部烟>上部烟;④混合型卷烟的总甾醇含量明显低于烤烟型;⑤同一类型的卷烟,国产卷烟的总甾醇含量较进口的低. The cholesterol, campesterol, stigmasterol and β - sitosterol in 15 samples of flue-cured tobacco leaves and 10 samples of cigarettes were determined. The samples were extracted with ace- tone, then hydrolyzed in 1% sulfuric acid of ethanol solution, and saponified in 2.0 mol/L potassium hydroxide of alcohol solution, reacted with trimethysilyl chloride and hexamethyldisilazane, and analyzed by capillary gas chromatography. The results showed that: 1 ) the recovery of these sterols ranged from 94.24% to 101.22% with RSD of 〈 5% ; 2) the contents of stigmasterol and sitosterol were the highest in all of the samples, no matter whether it was tobacco leaves or cigarette of Virginia type or blended type; followed by campesterol, and cholesterol was the lowest; 3) the total sterols in tobacco leaves from the same producing area decreased with the elevation of stalk position; 4) the total sterols in Virginia type cigarette was higher than that in blended type one; 5) for the same type cigarette, the total sterols in domestic cigarette was lower than that in imported one.
作者 张峻松 徐如彦 薄云川 王刘东 ZHANG JUN-SONG, XU RU-YAN, BO YUNC-HUAN, WANG LIU-DONG(1. School of Food and Bioengineening, Zhengzhou Institute of Light Industry, Zhengzhou 450002, China; 2. Xuzhou Cigarette Factory, Xuzhou 221005, Jiangsu, China) 
出处 《烟草科技》 EI CAS 北大核心 2007年第8期 27-31,共5页 Tobacco Science & Technology
关键词 烟草 甾醇 硅醚化 气相色谱法 Tobacco Sterol Trimethylsilylation Gas chromatography
作者简介 张峻松(1971-),博士,郑州轻工业学院副教授,硕士研究生导师,主要从事烟草化学研究.E-mail:zhangjs3@xinhuanet.com 电话:0371-63670231
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