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新型三齿多吡啶钴(Ⅱ)、iT(Ⅱ)配合物的合成、表征及其与DNA的作用研究 预览 被引量:5

Synthesis, Characterization and DNA-Binding Studies of New Tridentate Polypyridyl Cobalt(Ⅱ) and Ruthenium(Ⅱ) Complexes
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摘要 合成了两种新型三齿多吡啶钴(Ⅱ)和钌(Ⅱ)的混配配合物[Co(T0lylTPy)(H2Bzimpy)]Cl2[TolylTPy=4’-对甲基苯基-2,2’:6’,2″-三联吡啶,H2Bzimpy=2,6-二(苯并咪唑-2)吡啶](A)和Ru(TolylTPy)(Bzimpy)(B).用元素分析,IR,^1H NMR等对它们进行了表征,测定了配合物B的晶体结构,用电子吸收光谱、荧光光谱等研究了配合物与小牛胸腺DNA(CTDNA)的相互作用及其对pBR322DNA的断裂作用.结果表明,配合物A和B与CTDNA的作用属静电结合.凝胶电泳实验说明配合物A在310am光辐射15min,可使超螺旋pBR322DNA断裂为开环缺口型和线型DNA. Two novel tridentate polypyridyl mixed-ligand complexes of cobalt(Ⅱ) and ruthenium(Ⅱ), [Co(TolylTPy)(H2Bzimpy)]Cl2 [TolylTPy = 4'-p-tolyl-2,2':6',2"-terpyridine, H2Bzimpy = 2,6-bis(benzimidazol-2-yl)pyridine] (A) and Ru(TolylTPy)(Bzimpy) (B) were synthesized and characterized by elemental analysis, IR and ^1H NMR. The crystal structure of complex B was determined. The interactions between the complexes and calf thymus DNA have been investigated by absorption and fluorescence spectroscopies. The photoactivated cleavage of pBR322 DNA by the complexes was studied. The spectrophotometric studies suggested that both complexes A and B bind to DNA by electrostatic interactions. The agarose gel electrophoresis showed that complex A converted supercoiled pBR322 DNA to nicked and linear DNA when it was irradiated at 310 nm for 15 min.
作者 杨浩 陈文涛 邱东方 包晓玉 行文茹 刘珊珊 YANG, Hao CHEN, Wen-Tao QIU, Dong-Fang BAO, Xiao-Yu XING, Wen-Ru LIU, Shan-Shan (Department of Chemistry, Nanyang Normal University, Nanyang 473061)
出处 《化学学报》 SCIE CAS CSCD 北大核心 2007年第24期 2959-2964,共6页 Acta Chimica Sinica
基金 河南省教育厅自然科学基金(No.2006150020)资助项目.致谢 华中师范大学化学学院孟祥高老师在晶体结构测定中给予了大力帮助,在此表示衷心的感谢.
关键词 三齿多吡啶配体 钴(Ⅱ) 钌(Ⅱ)配合物 DNA 电子吸收光谱 荧光光谱 tridentate polypyridyl ligand cobalt(Ⅱ), ruthenium(Ⅱ) complex DNA absorption spectra fluorescence spectra
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