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Cd对小白菜生长及氮素代谢的影响研究 预览 被引量:32

Effects of Cadmium on the Growth and Nitrogen Metabolism in Brassica chinensis
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摘要 采用水培的方法,研究了不同Cd^2+水平(0、1、2.5、5、10 mg·L^-1)对小白菜叶片中铵态氮、硝态氮、可溶性蛋白质、游离脯氨酸、叶绿素、部分营养元素含量以及蛋白水解酶、硝酸还原酶、谷氨酰胺转化酶与合成酶活性的影响.结果表明,低浓度的Cd处理(1 mg·L^-1)刺激了小白菜的生长,提高了小白菜的生物量、叶绿素含量以及硝酸还原酶、谷氨酰胺合成酶与转化酶活性.Cd处理降低了小白菜对Cu、Ca、Fe、Mg的吸收,但促进了P的吸收.10 mg·L^-1的Cd处理显著降低了可溶性蛋白质含量、硝酸还原酶、谷氨酰胺合成酶和转化酶活性(p〈0.05),提高了蛋白水解酶活性,不利于叶片中铵态氮与硝态氮的同化,造成叶片中铵态氮和硝态氮的累积.小白菜叶片中游离脯氨酸含量与铵态氮含量成极显著正相关(p〈0.01),说明小白菜叶片中游离脯氨酸的累积在一定程度上缓解了铵的毒害. Hydroponic culture was conducted to study the effect of Cd on the growth, metal accumulation and nitrogen metabolism in Brassica chinensis. The enzymatic activities of nitrogen metabolism including nitrate reductase (NR), glutamine synthetase (GS) and GS-transferase as well as the concentrations of chlorophyll, free proline, soluble protein, NO3^- -N, NH4^+ -N and nutrients in Brassica chinensis were determined. Results indicated that the addition of Cd reduced the content of the soluble protein and the accumulation of Cu, Ca, Fe and Mg, but promoted the P uptake. Low level of Cd ( 1 mg· L^-1 ) could significantly increase the blomass and the content of chlorophyll of Brassica chinensis and the activities of NR, GS and GS-transferase when compared to control plants. However, when the Cd levels were above 2.5 mg· L^-1 in the culture medium, the activities of these enzymes were inhibited. Accordingly, the contents of NO3^--N, NH4^+-N, free proline and the activity of protease in the leaf of Brassica chinensis increased significantly. These results suggested that Cd addition could interfere with the assimilation of N in Brassica chinensis. The increase of free proline might alleviate the toxicity of ammonium in Brassica chinensis.
作者 于方明 仇荣亮 汤叶涛 应蓉蓉 周小勇 赵璇 胡鹏杰 曾晓雯 YU Fang-ming, QIU Rong-liang, TANG Ye-tao, YING Rong-rong, ZHOU Xiao-yong, ZHAO Xuan, HU Peng-jie, ZENG Xiao-wen (1 .School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China; 2. Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275, China; 3. School of Resource and Environment, Guangxi Normal University, Guilin 541004, China)
出处 《环境科学》 CAS CSCD 北大核心 2008年第2期 506-511,共6页 Chinese Journal of Environmental Science
基金 国家自然科学基金项目(40571144) 广东省自然科学基金重点项目(05101824) 广东省自然科学基金团队项目(06202438) 教育部“新世纪优秀人才支持计划”项目(NCET-04-0790) 教育部“985”二期项目 广西师范大学博士启动基金项目
关键词 CD 小白菜 氮素代谢 酶活性 Cd Brassica chinensis nitrogen metabolism enzymatic activity
作者简介 于方明(1975-),男,博士研究生,主要研究方向为水土环境污染生物修复,E-mail:fmyu1215@163.com 通讯联系人,E-mail:eesqrl@mail.sysu.edu.cn
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