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海昆肾喜对阿霉素肾病大鼠肾组织内皮素1及其mRNA表达和肾小球系膜细胞增殖的影响 预览 被引量:6

Effect of Haikun Shenxi Capsule on Endothelin-1 and mRNA Expression in Kidney Tissue of Rats with Adriamycin-induced Nephro- sis and Proliferation of GIomerular Mesangial Cells
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摘要 目的探讨海昆肾喜对阿霉素肾病大鼠内皮素1及其mRNA表达的影响以及对体外培养肾小球系膜细胞增殖的影响。方法将大鼠随机分为6组、正常组、假手术组、模型组、苯那普利组、海昆肾小剂量组及海昆肾喜大剂量组,10周后采用免疫组织化学及原位杂交方法分析肾组织内皮素1蛋白及mRNA表达情况;以苯那普利为实验对照组,采用MTT比色法测定海昆肾喜对肾小球系膜细胞增殖的影响。结果模型组大鼠肾组织中内皮素1及mRNA表达强阳性;海昆肾喜大、小剂量组较模型组表达显著减少(P〈0.05或P〈0.01);并且海昆肾喜可以明显抑制肾小球系膜细胞的增殖。结论海昆肾喜能够减少阿霉素肾病大鼠肾组织中内皮素1及mRNA的表达,抑制肾小球系膜细胞的增殖,从而延缓肾衰竭的的病程进展。 Objective To study the effect of Haikun Shenxi Capsule(HSC) on endothelin-1 (ET-1) and mRNA expression of rats with adriamycin-induced nephrosis and proliferation of glomerular mesangial cells (GMCs) cultured in vitro. Methods The rats were randomized into 6 groups: normal group, sham operation group, model group, benazepril group, HSC low-and high-dose groups. Ten weeks after treatment, ET-1 and mRNA expression were measured with immunohistochemical and hybridization in situ method. Tak- ing the Benazepril group as the control group, the proliferation of GMCs was detected with MTT method. Results In the model group, ET-1 and mRNA expression presented a strong positive, compared with which, that of the HSC low- and high-dose groups was remarkably reduced (P~0.05), and the effect of high-dose group was better as the proliferation of GMCs was remarkably suppressed. Conclusion HSC reduces remarkably ET-1 and mRNA expression in kidney tissue of rats with adriamycin-induced nephrosis and decreases the proliferation of GMCs, slowing down the development of renal failure.
作者 刘昆 赵宗江 张新雪 杨美娟 LIU Kun, ZHAO Zong-jiang, ZHANG Xin-xue, et al. ( Beijing University of Chinese Medicine, Beijing 100029)
出处 《中医杂志》 CSCD 北大核心 2008年第5期 456-458,共3页 Journal of Traditional Chinese Medicine
基金 教育部“长江学者和创新团队发展计划”资助项目(IRT0413) 国家自然科学基金藿点资助项目(30130220) 高等学校学科创新引智计划资助项目(B07007) 北京市中医管理局“51510”资助项目(JJ2007-006)
关键词 海昆肾喜 阿霉素肾病 大鼠 内皮素1 细胞增殖 Haikun Shenxi Capsule Adriamycin-induced nephrosis Rats Endothelin-1 (ET-1) Proliferation
作者简介 通讯作者:赵宗江,zongjiangz@sina.com,(010)64286988
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