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利血平致大鼠脾虚模型尿D-木糖排泄率与肠黏膜三磷酸腺苷水平的研究 被引量:17

Study on urine's xylose excretion rate and ATP in mucosa of spleen deficiency rats induced by reserpine
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摘要 [目的]观察利血平致脾虚大鼠的病理改变情况,为在该模型上观察益气健脾方药的作用提供参考。[方法]模型组以利血平注射造模15 d,停药后再观察30 d,实验周期共45 d;另设正常组观察45 d。观察大鼠一般状态、体重、尿D-木糖排泄率、小肠黏膜三磷酸腺苷(ATP)水平、胃肠黏膜病理组织学、脏器系数等指标。[结果]模型大鼠体重和尿D-木糖排泄率与正常组比较差异有统计学意义(P〈0.01),持续至45 d;小肠黏膜ATP水平比正常组略为降低;尿D-木糖排泄率改变与小肠黏膜ATP水平改变呈正相关;胃肠黏膜病理组织学检查未见明显改变;肝和胸腺脏器系数降低。[结论]利血平所致脾虚大鼠病变状态可持续较长时间,与益气健脾方药缓慢起效的特点相符;有肠道消化吸收功能降低的改变,并伴随肠黏膜ATP水平的降低。该动物模型可用于观察益气健脾方药改善消化吸收功能等作用。 [Objective]To observe the pathological changes and D-xylose excretion rate, ATP content in the small intestines mucosa membranes of rats with spleen deficiency induced by reserpine injection to provide reference for study on formula and herbs of strengthening spleen. [Methods]Spleen deficiency rat model was made by Reserpine injection for 15 days, and then continue observing 30 days, comparing with normal group. General conditions, body weight, D-xylose excretion rate, ATP content and histopathological study in mucosa were measured, [Results] The body weight and D-xylose excretion rate decreased after reserpine injection (P〈0. 01 ,vs. control group). The mucosal ATP content of model rats appreciably decreased, there was obvious positive correlation between change of urine's D-xylose excretion rate and mucosa ATP content. No obviously change was detected in histopathologic examination. The organ coefficient of liver and thymus were lower than that of normal rats. [Conclusion ]Pathogenic state of spleen deficiency rat caused by reserpine injection can last a long period, which is suitable for the characteristic of Chinese herb and formula for strengthening spleen. The model rats have dysfunction of digesting and absorbing, associated with mucosal ATP contents decreasing. So it can be used for studying Chinese herb and formula for strengthening spleen.
作者 郭文峰 高小玲 李茹柳 陈蔚文 GUO Wen- feng , GAO Xiao-ling , LI Ru-liu, CHEN Wei-wen(1. Piwei Institute,Guangzhou ;2. E-Institute of Shanghai of Traditional University of Chinese Medicine,Guangzhou Municipal Education Commission, Shanghai Chinese Medicine,Shanghai 201203 ,China) 510405 ,China; University
出处 《中国中西医结合消化杂志》 CAS 2008年第4期211-214,共4页 Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金 国家自然科学基金项目(30600798) 广东省自然科学基金项目(530048) 上海市教育委员会E-研究院建设计划项目(E03008)
关键词 脾虚证 动物模型 病理改变 尿D-木糖排泄率 三磷酸腺苷 spleen deficiency, animal model pathological changes urine's xylose excretion rate adenosine triphosphate
作者简介 郭文峰(1975-),男,江西于都人,医学博士,副教授,主要从事中西医结合消化道药理与临床研究 通讯作者:陈蔚文,Email:piwei@gzhtcm.edu.cn
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