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海绵来源放线菌sh6004发酵产物中的活性生物碱 预览 被引量:2

Bioactive alkaloids from sponge-derived actinomyces sh6004
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摘要 目的对一株来源于胶州湾海绵的放线菌sh6004发酵产物中的抗肿瘤活性成分进行分离和鉴定。方法采用溶剂萃取、硅胶柱色谱及制备HPLC等分离手段对该菌株发酵产物的活性部位进行了活性追踪分离,通过理化性质及波谱学手段进行化学结构鉴定,以SRB法和滤纸片法分别评价了化合物的抗肿瘤和抗菌活性。结果与结论从发酵产物中分离得到4个生物碱类化合物,其结构分别鉴定为bisdethiobis(methylthio) gliotoxin(1),放线菌素D(2),放线菌素C2a(3),放线菌素C3(4)。放线菌素是该菌株的主要活性成分,在浓度为10μg·mL^-1)时,化合物1~4表现出较强的抗金黄色葡萄球菌和抗枯草芽孢杆菌作用。 Objective To isolate and identify the antitumor components of the fermentation of the actinomyces sh6004 derived from a piece of sponge (unidentified) collected in Jiaozhou Bay. Methods The antitumor components were isolated by bioassay-guided fractionation and using solvent extraction, silica gel column chromatography and preparative HPLC. Their structures were established by physieochemical properties and spectral analyses. The cytotoxicity of compound 4 and the antimicrobial activities of compounds were evaluated by SRB and paper disc method, respectively. Results and Conclusion Four alkaloid compounds were isolated and identified as bisdethiobis- (methylthio)-gliotoxin(1), actinomycin D (2), actinomycin C2; (3)and actinomycin C3 (4), respectively. Actinomycins were the main antitumor components produced by actinomyces sh6004. Compounds 1-4 showed antimicrobial activities against Staphylococcus aureus and Bacillus subtilis at a concentration of 10ug· mL^-1.
作者 付海超 赵文英 钟惠民 洪葵 顾谦群 朱伟明 FU Hai-chao, ZHAO Wen-ying, ZHONG Hui-min, HONG Kui, GU Qian-qun, ZHU Wei-ming (1. Key Laboratory of Marine Drugs of MEC, School of Medicine and Pharmacy, China Ocean University, Qingdao 266003, China; 2. Qingdao University of Science and Technology, Qingdao 266042, China ;3. Institute of Tropical Biological Sciences and Biotechnology , Chinese Academy of Tropical Agricultural Science, Haikou 571101, China)
出处 《中国海洋药物》 CAS CSCD 2008年第4期 14-20,共7页 Chinese Journal of Marine Drugs
基金 海洋药物教育部重点实验室开放基金(KLMD-OUC-200607),国家863计划(2007AA092447)
关键词 海洋放线菌 抗菌 抗肿瘤 生物碱 放线菌素 spong-derived actinomyces antimicrobial antitumor alkaloids actinomycins
作者简介 付海超,男,硕士研究生 通讯作者:Tel:0532-82032065;fax:0532-82033054;E-mail:weimingzhu@ouc.edu.cn
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