目的对ImmunoCAP 250^TM检测过敏原特异性IgE抗体进行临床评价。方法依据美国国家临床实验室标准化委员会颁布的标准化文件，进行ImmunoCAP 250^TM检测过敏原特异性IgE抗体的精密度评价。将ImmunoCAP 250^TM和UniCAP 100的检测结果进行比较，分析其相关性。结果ImmunoCAP 250^TM对过敏原特异性IgE的检测有较好的精密度，批内、日间及总变异系数均小于6％。ImmunoCAP 250^TM和UniCAP 100对常见过敏原特异性IgE和总IgE的检测均有良好的相关性，相关系数分别为0．996和0．998。结论ImmunoCAP 250^TM检测过敏原特异性IgE的精密度高，与UniCAP 100比较相关性好，检测通量大，自动化程度高，适于大中型实验室的临床应用．
Objective To evaluate the clinical pecision of ImmunoCAP 250TM for testing allergen specific IgE antibody. Methods Precision was evaluated according to the guideline issued by the National Committee on Clinical Laboratory Standards （NCCLS）, and the correlation between ImmunoCAP 250TM and UniCAP 100~ was analyzed. Results ImmunoCAP 250TM was precise for testing allergen specific IgE antibody, having a within-run CV 2.96%, day-to-day CV 4.69%, total CV 5.36%. Allergen specific IgE and total IgE values measured by ImmunoCAP 250TM correlated well with those by UniCAP 100, the correlation coefficients were 0.996 and 0.998, respectively. Conclusions ImmunoCAP 250TM is precise and correlated well with UniCAP 100 for testing allergen specific IgE antibody. ImmunoCAP 250TM is highly automatic and easy of handling with massive samples, and clinically useful.
Chinese Journal of Allergy & Clinical Immunology