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咔唑分光光度法测定猪硫酸软骨素含量 预览 被引量:9

Content Determination of Chondroitin Sulfate in Pig by Charbazole Spectrophotometry
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摘要 [目的]建立咔唑分光光度法测定猪硫酸软骨素含量的方法。[方法]利用咔唑分光光度法,在浓硫酸介质中,将猪硫酸软骨素的标准品和样品溶液经咔唑显色反应,在λ为518nm处分别测定其吸光度4,按标准曲线法计算含量。[结果]猪硫酸软骨素质量浓度在0~0.25g/L范围内线性关系良好,相关系数r=0.9998(n=7),加样回收率为99.48%,RSD为0.73。[结论]该测定方法简单、快速,结果准确,可用作猪硫酸软骨素含量的测定方法。 [Objective] The research aimed to establish the determination method of the content of chondroitin sulfate in pig by earbazole spectropho- tometry. [ Method] The color reaction of carbazole was made on the standard substance and samples of pig chondroitin sulfate in concentrated sulphuric acid. Its absorbency was determined at λ= 518 nm by using carbazole spectrophotometry and its content was calculated by using the standard curve method. [Result] A good lincar relationship was shown in the mass concentration range of pig chondroitin sulfate (0- 0.25 g/L). The correlation coefficient (r) was 0.999 8 ( n = 7). The recovery rate of added samples was 99.48 %, with RSD of 0.73, [ Conclusion ] The determination method is simple, rapid and accurate. And it can be used as the determination method of chondroitin sulfate content in pig.
作者 陆钊 杨亚楠 LU Zhao et al (Pharmacy College,Jilin Medical College,Jilin,Jilin 132013)
出处 《安徽农业科学》 CAS 北大核心 2008年第33期 14368,14371,共2页 Journal of Anhui Agricultural Sciences
关键词 咔唑法 猪硫酸软骨素 测定 Carhazole inethod Pig chondroitin sulfate Determination
作者简介 陆钊(1972-),男,黑龙江哈尔滨人,在读硕士,讲师,从事高分子材料工艺研究. 通讯作者,博士,硕士生导师,副教授.
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