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产植酸酶的黑曲霉菌株筛选及其植酸酶基因克隆 预览 被引量:46

Islation of Aspergillus niger 963 withPhytase Activity and Cloning Its Phytase Gene phy A2
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摘要 从土壤中筛选到产植酸酶的黑曲霉菌株Aspergillus niger 963,对其所产植酸的生理化性质研究表明其具有适合于在饲料中使用的优良性质它的酶活性最适温度为55℃,最适PH值分别为pH1.8和5.7,并且pH1.8-5.7的范围内,植酸酶均能维持较高的酶活性。 Aspergillus niger 963 secreting phytase is isolated from soil . It is cultivated by a shaking method and phytase is pro - duced 165 U/mL . The phytase shows following properties . a ) Optimal pH values of the phytase for its activity are 1. 8 and 5 . 7 . b) Optimal temperature of the phytase for its activity is about 55 ℃ . The gene phy A2 encoding the phytase is cloned from A. niger 963 by polymerase chain reaction( PCR) . Nucleotide sequence analysis of phy A2 revealed that the presence of an open reading frame cod- ing for 467 amino acids and interrupted once by an intron of 102 bp in the 5' put of gene . The start codon is followed by a sequence coding for a putative signal peptide ( 19 amino acids in length ) . There are nine potential N - glycosylation sites in phytase . Compari- son with phytase gene phyA from A . niger ( ficuum) var . awamori shows 91 . 8% homology in nucleotides and 91. 6% in amino acids residues .
作者 姚斌 张春义 Yao Bin Zhang Chunyi Wang Jianghua Fan YunliuShi xiuyun wang Yaru Li shuming(1Feed Research Institute,Chinese Academy of Agricultural Scices,Beijing 100081;2 Biotechnology Research Center, Chinese Academy of Agricultural Sciences,Beijing 100081)
出处 《农业生物技术学报》 CAS 1998年第1期 1-6,共6页 Journal of Agricultural Biotechnology
基金 国家高技术研究与发展计划(“863”计划)资助
关键词 植酸酶 菌株筛选 植酸酶基因 克隆 饲料 : phytase Aspergillus niger 963 pyy A2
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参考文献1

  • 1Yi Z,Anim Feed Sci Technol,1996年,61卷,361页 被引量:1

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