Bone tissue engineering should take much advantages of beta-tricalcium phosphate (β-TCP) due to its good bone conductivity and biocompatibility. However, porousβ-TCP cell scaffolds do not meet the requirements of bone tissue engineering for lacking of strength and interconnection. Purpose To improve the interconnection and strength ofβ-TCP scaffolds, a newly additive and preparative technique was studied in this paper. Methods Pureβ-TCP powder was obtained by sinteringβ-TCP precursor, which was synthesized with a new method called two stages reaction through trickling Ca(OH)2 suspensions to H3PO4 solution by controlling Ca to P molar ratio 1.5; Then theβ-TCP powder was mixed with porogen which composed of stearic acid and paraffin spheres coated with liquid hydrocarbon. Added K2HPO4 solution into the mixture and blend homogeneously to obtain slurry, pressed the slurry in a mold to getβ-TCP flan, dried and sintered theβ-TCP flan to harvest porous scaffolds, immersed in distilled water to remove the product of K2HPO4 to get scaffolds. Finally, the pore structure and the properties of the scaffolds were characterized. Results The results showed that the chemical substance of scaffolds wasβ-TCP, the pores were interconnected and homogeneously distributed, and average pore size was 781.3±70.47 (n=12)μm and diameter of the channel between pores was 297.88±66.86 (n=13)μm, respectively. Porosity, water uptake, compressive strength of scaffolds were 52.27±0.11 (n=6)Vol%, 31.82±0.13(n=6)Wt%, 11.40±0.07(n =6)MPa, respectively. Conclusionβ-TCP powder could be obtained by two stages neutralization reaction;β-TCP cell scaffolds with homogeneous and controllable pore structure high strength and interconnection could be fabricated by our invented method. And the scaffolds have potential application in bone tissue engineering.
Journal of Tissue Engineering and Reconstructive Surgery