期刊文献+

高孔隙连通性β-磷酸三钙细胞支架的制备 预览 被引量:4

Preparation of Porous β-tricalcium Phosphate Scaffold with High Interconnection
在线阅读 下载PDF
收藏 分享 导出
摘要 目的改进多孔β-TCP支架的制备方法,提高支架的孔隙连通性、孔隙结构的均匀性以及支架的抗压特性。方法利用两阶段中和反应工艺,制备β-TCP粉末原料;将粘结剂均匀涂布在致孔剂表面后与β-TCP粉末混合,添加高温液相传质介质,再次混合形成致孔剂与β-TCP粉末的均匀混合体,加压成型、煅烧制备三维多孔细胞支架;X-衍射检测原料和支架的成分,扫描电镜观察支架的孔隙结构,力学实验仪测定支架的抗压性能。结果原料和支架化学成分均为β-TCP;支架的孔隙呈球形、分布均匀、孔隙间几乎完全连通,大孔平均孔径781.38±70.47(n=12)μm,连通孔径297.88±66.86(n=13)μm;孔隙率、吸水率和抗压强度分别为52.27±0.11(n=6)Vol%、31.82±0.13(n=6)Wt%和11.40+0.07(n= 6)MPa。结论两阶段中和反应工艺能够制备出纯的β-TCP粉末,改进的支架制备技术,可以制备出孔隙率高、强度大、孔隙大小可控、孔隙分布均匀、孔隙间几乎完全连通的β-TCP支架,具备了组织工程要求的结构特征。 Bone tissue engineering should take much advantages of beta-tricalcium phosphate (β-TCP) due to its good bone conductivity and biocompatibility. However, porousβ-TCP cell scaffolds do not meet the requirements of bone tissue engineering for lacking of strength and interconnection. Purpose To improve the interconnection and strength ofβ-TCP scaffolds, a newly additive and preparative technique was studied in this paper. Methods Pureβ-TCP powder was obtained by sinteringβ-TCP precursor, which was synthesized with a new method called two stages reaction through trickling Ca(OH)2 suspensions to H3PO4 solution by controlling Ca to P molar ratio 1.5; Then theβ-TCP powder was mixed with porogen which composed of stearic acid and paraffin spheres coated with liquid hydrocarbon. Added K2HPO4 solution into the mixture and blend homogeneously to obtain slurry, pressed the slurry in a mold to getβ-TCP flan, dried and sintered theβ-TCP flan to harvest porous scaffolds, immersed in distilled water to remove the product of K2HPO4 to get scaffolds. Finally, the pore structure and the properties of the scaffolds were characterized. Results The results showed that the chemical substance of scaffolds wasβ-TCP, the pores were interconnected and homogeneously distributed, and average pore size was 781.3±70.47 (n=12)μm and diameter of the channel between pores was 297.88±66.86 (n=13)μm, respectively. Porosity, water uptake, compressive strength of scaffolds were 52.27±0.11 (n=6)Vol%, 31.82±0.13(n=6)Wt%, 11.40±0.07(n =6)MPa, respectively. Conclusionβ-TCP powder could be obtained by two stages neutralization reaction;β-TCP cell scaffolds with homogeneous and controllable pore structure high strength and interconnection could be fabricated by our invented method. And the scaffolds have potential application in bone tissue engineering.
作者 安身平 陈际达 张永红 AN Shenping,CHEN Jida, ZHANG Yonghong. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 40044, China
出处 《组织工程与重建外科》 2005年第6期323-326,共4页 Journal of Tissue Engineering and Reconstructive Surgery
关键词 多孔β-TCP 细胞支架 骨组织工程 孔隙连通 Porousβ-TCP Cell scaffolds Bone tissue engineering Interconnection pore
  • 相关文献

参考文献2

二级参考文献2

共引文献14

同被引文献28

引证文献4

二级引证文献2

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈
新型冠状病毒肺炎防控与诊疗专栏