期刊文献+

两种不同纯度的嗅鞘细胞在体外存活与生长的比较 被引量:1

Comparison of the cellular survival and growth between olfactory ensheathing cells of two different purities in vitro
收藏 分享 导出
摘要 比较80%和50%两种纯度的嗅鞘细胞在体外培养条件下的存活与生长,为嗅鞘细胞体内移植选用最佳纯度提供依据。分离、培养并纯化成年SD大鼠嗅鞘细胞,将纯化的嗅鞘细胞和收集到的贴壁成纤维细胞进行约80%和50%的比例混合后接种,继续培养1d或3d。所有细胞于不同时间点行P75免疫细胞化学荧光染色,以鉴定嗅鞘细胞的初始及其后培养过程中数量和纯度的变化。观察细胞状态,计数细胞总数和P75免疫阳性细胞数目,求得各组嗅鞘细胞数量和纯度并行统计学分析。结果显示:两种不同纯度嗅鞘细胞在培养1d时形态正常,3d时纯度为50%的嗅鞘细胞胞体依然饱满,突起更加纤长,数量和纯度亦无明显下降。而纯度为80%的嗅鞘细胞在培养3d时已出现胞体萎缩和突起变短,数量从1d时每一观察区域内的35±13显著下降为23±5(P=0.02),但纯度未发生明显变化。结果表明50%纯度嗅鞘细胞的存活及生长状态优于80%者,提示细胞体内移植时应考虑选取50%纯度的嗅鞘细胞。 In order to compare the survival and growth of olfactory ensheathing cells (OECs) of 80% and 50% purities in vitro, and to present the evidence for the optimal purity of OECs for the transplantation, OECs of adult SD rats were isolated, cultured, purified and mixed for a purity of approximate 80% or 50% with attached fibroblasts. Mixed ceils were cultured for one or three days. All the cultured cells were stained with anti-p75 immunocytochemistry to verify the initial and subsequent numbers and purities of OECs during the cell culture. The morphology of OECs and fibroblasts were observed, the numbers of the total cells and the p75-positive cells were counted, then the purities of OECs were obtained. The mean numbers and purities of the p75-positive cells were analysed statistically. Our results indicated that the OECs with 80% and 50% purities 1 day after culture showed the normal morphology. When the culture time increased to 3 days, OECs with 50% purity had plump cell bodies and long processes, and the mean number and purity of surviving OECs had no dramatical decrease, in comparison with those at 1 day. However, OECs of 80% showed the shrinkage in cell body and the shorter processes, and the mean number of surviving OECs per observing area decreased remarkedly from 35 ± 13 to 23 ± 5 (P = 0.02), when compared with those at 1 day. We also noted that there was no change in the purity of OECs between 1 and 3 day time points. The present results indicate that the survival and growth of cultured OECs of 50% purity are predominant to those of OECs of 80%, suggesting that 50% OEC suspension should be an optimal candidate for the transplantation into the injured central nervous system.
作者 王涛 杨浩 罗娜 武明媚 焦西英 游思维 Wang Tao, Yang Hao ,Luo Na, Wu Mingmei, Jiao Xiying, You Siwei (Institute of Neurosciences, the Fourth Military Medical University, Xi'an 710032)
出处 《神经解剖学杂志》 CAS CSCD 北大核心 2009年第5期541-545,共5页 Chinese Journal of Neuroanatomy
基金 国家自然科学基金(30571998,30872829)资助项目
关键词 嗅鞘细胞 成纤维细胞 细胞培养 细胞存活 细胞生长 Olfactory ensheathing cell, fibroblast, cell culture, cell survival, cell growth
作者简介 通讯作者 游思维 电话:029—84776755 E-mail:yousiwei@fmmu.edu.cn
  • 相关文献

参考文献21

  • 1Ramon-Cueto A, Avila J. Olfactory ensheathing glia: properties and function. Brain Res Bull, 1998;46:175-187. 被引量:1
  • 2Verdu E, Garcia-Alias G, Fores J et al. Olfactory ensheathing cells transplanted in lesioned spinal cord prevent loss of spinal cord parenchyma and promote functional recovery. Glia, 2003 ;42:275- 286. 被引量:1
  • 3Wang YZ, Meng JH, Yang H et al. Differentiation-inducing and protective effects of adult rat olfactory ensheathing cell conditioned medium on PC 12 cells. Neurosei Lett,2003 ; 346:9-12. 被引量:1
  • 4Sasaki M, Hains BC, Lankford KL et al. Protection of corticospinal tract neurons after dorsal spinal cord transection and engraftment of olfactory, ensheathing cells. Glia,2006 ;53 :352-359. 被引量:1
  • 5Shyu WC, Liu DD, Lin SZ et al. Implantation of olfactory ensheathing cells promotes neuroplasticity in murine models of stroke. J Clin Invest,2008 ; 118 : 2482-2495. 被引量:1
  • 6Li Y, Sauve Y, Li D et al. Transplanted olfactory ensheathing cells promote regeneration of cut adult rat optic nerve axons. J Neurosci, 2003 ;23:7783-7788. 被引量:1
  • 7Choi D, Li D, Law S et al. A prospective observational study of the yield of olfactory ensheathing cells cultured from biopsies of septal nasal mucosa. Neurosurgery,2008 ;62 : 1140-1144. 被引量:1
  • 8Richter MW, Roskams AJ. Olfactory ensheathing cell transplantation following spinal cord injury: hype or hope. Exp Neurol,2008; 209 : 353-367. 被引量:1
  • 9Teng X, Nagata I, Li HP et al. Regeneration of nigrostriatal dopaminergic axons after transplantation of olfactory ensheathing cells and fibroblasts prevents fibrotic scar formation at the lesion site. J Neurosci Res, 2008 ;86:3140-3150. 被引量:1
  • 10Ramon-Cueto A, Plant GW, Avila J et al. Long-distance axonal regeneration in the transected adult rat spinal cord is promoted by olfactory ensheathing glia transplants. J Neurosci, 1998 ; 18 : 3803- 3815. 被引量:1

二级参考文献15

  • 1[1]Verdu E, Narvarro X, Gudino Cabrera G, et al. Olfactory bulb ensheathing cells enhance peripheral nerve regeneration [J]. Neuroreport, 1999,10(5):1097. 被引量:1
  • 2[2]Imaizumi T, Lankford KL, Kocsis JD. Transplantation of olfactory ensheathing cells or schwann cells restores rapid and secure condition arcoss the transected spinal cord [J]. Brian Res, 2000,854(1-2):70. 被引量:1
  • 3[3]Ramon-cueto A, Cordero MI, Santos-Benito FF, et al. Functional recovery of paraplegic rats and motor axon regeneration in their spinal cords by olfactory ensheathing glia [J]. Neuron, 2000,25(2):425. 被引量:1
  • 4[4]Imaizumi T, Lankford KL, Kocsis ID, et al. Comparison of myelin-forming cells as candidates for therapeutic transplantation in demyelinated CNS axons No To Shinkei, 2000,52(7):609. 被引量:1
  • 5[5]Sonigra RJ, Brighton PC, Jacoby J, et al. Adult rat olfactory nerve ensheathing cells are effective promoters of adult central nerves system neurite outgrowth in coculture [J]. Glia, 1999,25(30:256. 被引量:1
  • 6[6]Li Y, Field PM, Raisman G, Repair of adult rat corticospinal tract by transplants of olfacory ensheathing cells [J]. Science, 1997,277(5334):2000. 被引量:1
  • 7[7]Ramon-Cueto A, Plant GW. Avila J, et al. Long-distance axonal regeneration in the transected adult rat spinal cord in promoted by olfactory ensheathing glia transplants [J]. J Neurosci, 1998,18(1):3803. 被引量:1
  • 8[8]Kott JN, Westrum LE, Raines EW, et al. Olfactory ensheathing glia and platelet-derived growth factor B-chain reactivity in the transplanted rat olfactory bulb [J]. Int J Dev Neurosci, 1994,12(4):315. 被引量:1
  • 9[9]Van Eldik LJ, Christie-pope B. Neurotrophic activity of S-100 beta in cultures of dorsal root ganglia from embryonic chick and fetal rat [J]. Brain Res, 1991,542(2):280. 被引量:1
  • 10[10]Tisay KT, Key B. The extracellular matrix modulates olfactory neurite outgrowth on ensheathing cells [J]. J Neurosci, 1999,19(22):9890. 被引量:1

共引文献18

同被引文献13

  • 1赵君朋,王珂,杨磊,雷季良.嗅成鞘细胞在新生大鼠嗅球的分布[J].解剖学杂志,2005,28(2):228-230. 被引量:3
  • 2Dressier MR, Butler DL, Boivin GP. Effects of age on the repair ability of mesenchymal stem cells in rabbit tendon [J]. J Orthop Res, 2005, 23:287 -289. 被引量:1
  • 3Nash HH, Borke RC, Anders JJ. New method of purification for es- tablishing primary cultures of ensheathing cells from the adult olfac- tory bulb[J]. Glia, 2001,34:81 -87. 被引量:1
  • 4Barnett SC, Hutchins AM, Noble M. Purification of olfactory nerve ensheathing cells from the olfactory bulb [J]. Dev Bio1,1993,155 : 337 - 350. 被引量:1
  • 5Ramon-Cueto A, Avila J. Olfactory ensheathing glia:properties and function [ J ]. Brain Res Bull, 1998, 46 : 175 - 187. 被引量:1
  • 6周长满 买鸿宴 杨立元等.大鼠嗅球和鼻腔粘膜成鞘细胞的形态学研究.懈剖学报,2003,34:11-14. 被引量:1
  • 7Chuah MI, West AK. Cellular and molecular biology of ensheating cells [ J ]. Microsc Res Tech, 2002, 58:216 - 227. 被引量:1
  • 8Doueette R. Glial progenitor cells of the nerve fiber layer of the ol- factory bulb: effect of astroeyte growth media[ J]. J Neurosci Res, 1993, 35 : 274 -287. 被引量:1
  • 9Lu J, Feron F, Ho SM, et al. Transplantation of nasal olfactory tis- sue promotes partial recovery in paraplegic adult rats [ J ]. Brain Res, 2001,889:344 - 357. 被引量:1
  • 10李玉安,徐华梓,李万里.单次差速贴壁法纯化培养大鼠嗅神经鞘细胞的实验研究[J].实用医学杂志,2009(10):1562-1565. 被引量:2

引证文献1

二级引证文献2

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈