In order to compare the survival and growth of olfactory ensheathing cells （OECs） of 80% and 50% purities in vitro, and to present the evidence for the optimal purity of OECs for the transplantation, OECs of adult SD rats were isolated, cultured, purified and mixed for a purity of approximate 80% or 50% with attached fibroblasts. Mixed ceils were cultured for one or three days. All the cultured cells were stained with anti-p75 immunocytochemistry to verify the initial and subsequent numbers and purities of OECs during the cell culture. The morphology of OECs and fibroblasts were observed, the numbers of the total cells and the p75-positive cells were counted, then the purities of OECs were obtained. The mean numbers and purities of the p75-positive cells were analysed statistically. Our results indicated that the OECs with 80% and 50% purities 1 day after culture showed the normal morphology. When the culture time increased to 3 days, OECs with 50% purity had plump cell bodies and long processes, and the mean number and purity of surviving OECs had no dramatical decrease, in comparison with those at 1 day. However, OECs of 80% showed the shrinkage in cell body and the shorter processes, and the mean number of surviving OECs per observing area decreased remarkedly from 35 ± 13 to 23 ± 5 （P = 0.02）, when compared with those at 1 day. We also noted that there was no change in the purity of OECs between 1 and 3 day time points. The present results indicate that the survival and growth of cultured OECs of 50% purity are predominant to those of OECs of 80%, suggesting that 50% OEC suspension should be an optimal candidate for the transplantation into the injured central nervous system.
Chinese Journal of Neuroanatomy
Olfactory ensheathing cell, fibroblast, cell culture, cell survival, cell growth
通讯作者 游思维 电话：029—84776755 E-mail：yousiwei@fmmu．edu．cn