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非甲状腺疾病大鼠中枢甲状腺功能及受体α1mRNA水平与认知能力的研究 被引量:1

Relationship between cognitive ability and both thyroid function and mRNA level of thyroid hormone receptor α1 in brain tissue of rats with nonthyroidal illness syndrome
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摘要 目的研究非甲状腺疾病综合征(NTIS)大鼠认知能力、中枢甲状腺功能及甲状腺激素受体α1(TRα1)mRNA表达的变化。方法19只成年SD大鼠按随机数字表法分为正常组(n=9)和NTIS组(n=10)。腹腔注射四氯化碳(CCl4,用玉米油按1:6稀释)0.15mL,使大鼠肝纤维化制作NTIS模型,正常组注射等量生理盐水。采用Morris水迷宫试验检测大鼠的记忆认知能力;放免法测定血清甲状腺激素(T1、T4、FL3、FT4、rT3、TSH)和脑组织甲状腺激素(T3、T4、rT3)浓度;采用荧光定量RT-PCR法测定脑组织中TRα1mRNA水平。结果2组大鼠定向航行试验及空间探索试验结果比较差异无统计学意SL(P〉0.05);NTIS组大鼠血清T3、T4、FT3、FT4浓度低于正常组,差异有统计学意义(P〈0.05);2组大鼠血清rT3、TSH浓度,脑组织T3、T4、rT3浓度,TRα1mRNA的表达水平比较差异均无统计学意义(P〉0.05)。结论肝纤维化大鼠具有典型的NTIS表现;NTIS大鼠认知能力、中枢甲状腺功能无明显异常,脑组织TRα1mRNA表达无明显变化。 Objective To explore the changes of cognitive ability and thyroid function and mRNA level of thyroid hormone receptor α1 (TRα1) in the brain tissues of rats with nonthyroidal illness syndrome (NTIS). Methods Nineteen adult male SD rats were randomly divided into normal control (n=9) and NTIS (n=10) groups. The NTIS model, its liver been fiberized was induced by intraperitoneal injecting 0.15 mL carbon tetrachloride ([CCL4], [1:6, diluted by corn oil]) 3 times per week with duration of 8 weeks. The normal control group was injected equal sodium. Morris water-maze task was employed to test the cognitive memory ability of the rats; the concentrations of T3, T4, FT3, FT4, rT3 and TSH in the blood serum and T3, T4 and rT3 in the brain tissue were detected by using radioimmunoassay (RIA); the mRNA level of TRα1 was detected by fluorescent quantitative RT-PCR. Results Morris water-maze task showed no significant difference in congnitive ability between NTIS and control groups (P〉0.05). The concentrations ofT4, FT3, FT4 and T3 in blood serum in the NTIS group were significantly lower as compared with those in the normal control group (P〈0.05). Concentrations of rT3, TSH in the blood serum, the concentrations of T4, T3, rT3 and the mRNA level of TRα1 in the brain tissue had no significant differences between the two groups (P〉0.05). Conclusion The rats with hepatic fibrosis showed typical NTIS. Thyroid function in central nervous system of these rats is normal, so is their cognitive ability. The mRNA level of TRα1 had no significant change in brain tissue of these rats.
作者 袁大华 王群 YUAN Da- hua, WANG Qun. (Department of Neurology, Nandang Hospital, Southern Medical University, Guangzhou 510515, China)
出处 《中华神经医学杂志》 CAS CSCD 北大核心 2010年第1期34-37,42共5页 Chinese Journal of Neuromedicine
关键词 非甲状腺疾病综合征 甲状腺激素 认知功能 甲状腺激素受体 Nonthyroidal illness syndrome Thyroid hormone Cognition Thyroid hormone receptor
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