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番木瓜PL和β-Gal基因双价反义表达载体的构建 预览 被引量:1

Construction of PL and β-Gal antisense expression vector of papaya
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摘要 在已报道的番木瓜果胶裂解酶(PL)和β-半乳糖苷酶(β-Gal)基因序列的基础上,设计特异引物,分别扩增保守区序列,将其分别反向插入植物表达载体pCAMB IA1301-35S-GUS-Nos的CaMV35S启动子和Nos终止子之间,构建反义表达载体pCP和pCG.然后用2种不同的方法将带有完整启动子和终止子的PL和-βGal基因引入pCAMB IA2301中,获得PL和β-Gal基因的双价植物反义表达载体pCPG,并对2种方法进行比较. Pectate lyase and β-Galactosidase(β-Gal) are important softening enzyme.The transcriptions of PL and β-Gal gene were inhibited by antisense technique may put off papaya(Carica papaya L.) fruit softening.PL and β-Gal conserved domains were cloned using specific primers designed according to those published genes.The PL and β-Gal cDNA were inserted into between the CaMV35S promoter and Nos terminator of the expression vector pCAMBIA1301-35S-GUS-Nos with reverse orientation,respectively.So,two antisense expression vectors pCP and pCG were obtained.Then,PL and β-Gal antisense genes with 35S promoter and Nos terminator were inserted into expression vector pCAMBIA2301 together to generate double genes plant expression vector pCPG by different mode.
作者 申艳红 陈晓静 何玮毅 SHEN Yan-hong,CHEN Xiao-jing,HE Wei-yi(College of Horticulture/Institute of Genetics and Breeding in Horticultural Plants,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
出处 《福建农林大学学报:自然科学版》 CSCD 北大核心 2010年第2期 159-163,共5页 Journal of Fujian Agricultural and Forestry University
基金 福建省自然科学基金资助项目(B0610010)
关键词 番木瓜 果胶裂解酶 Β-半乳糖苷酶 反义表达载体 papaya pectate lyase β-Galactosidase antisense expression vector
作者简介 申艳红(1978-),女,讲师,博士研究生.研究方向:园艺植物遗传育种. 通讯作者陈晓静(1954-),女,教授.研究方向:园艺植物遗传育种.Email:xjchen804@sina.com.
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