期刊文献+

乳腺癌HER2与TOP2A的表达及其相关性 被引量:4

Expression level and amplification condition of HER2 and TOP2A as well as their relationship in breast cancer
收藏 分享 导出
摘要 目的 探讨乳腺癌患者HER2与TOP2A基因及蛋白的表达情况及其表达的相关性. 方法 采用免疫组织化学方法与荧光原位杂交(FISH)方法,检测58例乳腺癌中HER2及TOP2A的基因及蛋白表达情况,并分析其相关性.结果 58例乳腺癌患者,HER2评分:0分11例(11/58,19.0%),1^+19例(19/58,32.8%),2^+13例(13/58,22.4%),3+15例(15/58,25.8%) TopoⅡα评分:0分28例(28/58,48.3%),1^+22例(22/58,37.9%),2^+8例(8/58,13.8%).HER2基因扩增19例(19/58,32.8%),HER2基因无扩增39例(39/58,67.2%) TOP2A基因扩增11例(11/58,19.0%),TOP2A基因无扩增47例(47/58,81.0%).免疫组织化学方法检测HER2与FISH检测HER2的结果明显相关,其相关系数rs=0.80(P〈0.05) 免疫组织化学方法检测TopoⅡα与FISH检测TOP2A结果相关,其相关系数rs=0.50(P〈0.05) FISH检测HER2与TOP2A的结果明显相关,相关系数rs=0.69(P〈0.05). 结论 HER2和TOP2A在乳腺癌中的扩增具有高度一致性,TOP2A的扩增及表达可能依赖于HER2的扩增及表达. Objective To investigate the expression level and amplification condition of HER2 and TOP2A as well as their relationship in breast cancer. Methods Immunohistochcmistry and fluorescent in situ hybridization (FISH) were taken to detect the the expression level of HER2/topo Ⅱα, amplification status of HER2 gene/TOP2A gene respectively. Statistical analysis was performed to explore the correlation between them. Results The breast cancer tissues of 58 patients were tested. The expression level of HER2 was investigated in 11 cases with negative expression( 11/58,19. 0% ) , 19 cases with weakly positive expression( 19/58,32.8% ) , 13 cases with positive expression( 13/ 58,22.4% ) and 15 cases with strongly positive expression( 15/58,25.8% ). The expression level of topo 1I ct was investigated in 28 cases with negative expression ( 28/58,48.3% ) , 22 cases with positive expression ( 22/58,37.9% ) , 8 cases with strongly expression ( 8/58, 13.8% ). Amplification of HER2 and TOP2A gene was detected in 19 patients ( 19/58,32.8% ) ,11 patients ( 11/58, 19.0% )respectively. Non-amplification of HER2 gene and TOP2A was detected in 39 patients (39/58,67.2%), 47 patients(47/58,81.0% )respeetively. Spearman rank correlation analysis revealed that the expression level of HER2 protein was significantly related with the amplification status of HER2 gene ( rs = 0.80, P 〈 0. 05 ) and the expression level of topo Ⅱα was moderately related with the amplification status of TOP2A gene (r = 0. 50, P 〈 0. 05). The correlation between the amplification status of HER2 gene and TOP2A gene was also significant( rs = 0. 69 ,P 〈 0.05 ). Conclusion The expression level of HER2 protein and the amplification status of HER2 gene correlate significantly with the expression level of topo Ⅱα, TOP2A gene respectively. The expression level and amplication status of TOP2A may rely on the expression level and amplication status of HER2 respectively.
作者 明洁 逯翀 田元 潘华雄 黄韬 刘春萍 MING Jie, LU Chong, TIAN Yuan, PAN Hua-xiong, HUANG Tao, LIU Chun-ping (Department of Breast and Thyroid Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China)
出处 《解剖学报》 CAS CSCD 北大核心 2010年第3期391-394,共4页 Acta Anatomica Sinica
基金 湖北省自然基金资助项目(2008CDB194)
关键词 乳腺癌 HER2 TOP2A 免疫组织化学 荧光原位杂交 Breast cancer HER2 TOP2A Immunohistochemistry Fluorescent in situ hybridization Human
作者简介 明洁(1981-),女(汉族),安徽省人,博士研究生。 通讯作者(To whom correspondence should be addressed)E-mail: lcp191@ 163. com Tel(027)85351622
  • 相关文献

参考文献8

  • 1Siddiqa A,Long LM,Li L,et al.Expression of HER2 in MCF-7 breast cancer cells modulates anti-apoptotic proteins Survivin and Bcl-2 via the extracellular signal-related kinase (ERK) and phosphoinositide-3 kinase (PI3K) signalling pathways[J].BMC Cancer,2008,5(8):129. 被引量:1
  • 2Sińczak-Kuta A,Tomaszewska R,Rudnicka-Sosin L,et al.Evaluation of HER2/neu gene amplification in patients with invasive breast carcinoma.Comparison of in situ hybridization methods[J].Pol J Pathol,2007,58(1):41-50. 被引量:1
  • 3Roca J.Topoisomerase II:a fitted mechanism for the chromatin landscape[J].Nucleic Acids Res,2009,37(3):721-730. 被引量:1
  • 4Knoop AS,Knudsen H,Balslev E,et al.Retrospective analysis of topoisomerase IIa amplifications and deletions as predictive markers in primary breast cancer patients randomly assigned to cyclophosphamide,methotrexate,and fluorouracil or cyclophosphamide,epirubicin,and fluorouracil:Danish Breast Cancer Cooperative Group[J].J Clin Oncol,2005,23(30):7483-7490. 被引量:1
  • 5Arriola E,Rodriguez-Pinilla SM,Lambros MB,et al.Topoisomerase II alpha amplification may predict benefit from adjuvant anthracyclines in HER2 positive early breast cancer[J].Breast Cancer Res Treat,2007,106(2):181-189. 被引量:1
  • 6Olsen KE,Knudsen H,Rasmussen BB,et al.Amplification of HER2 and TOP2A and deletion of TOP2A genes in breast cancer investigated by new FISH probes[J].Acta Oncol,2004,43(1):35-42. 被引量:1
  • 7Jarvinen TA,Tanner M,Rantanen V,et al.Amplification and deletion of topoisomerase IIα associate with ErbB-2 amplification and affect sensitivity to topoisomerase II inhibitor doxorubicin in breast cancer[J].Am J Pathol,2000,156(3):839-847. 被引量:1
  • 8Liang Z,Zeng X,Gao J,et al.Analysis of EGFR,HER2,and TOP2A gene status and chromosomal polysomy in gastric adenocarcinoma from Chinese patients[J].BMC Cancer,2008,8:363. 被引量:1

同被引文献14

引证文献4

二级引证文献9

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈