A HPLC method for the determination of pyraclostrobin residues in grape and soil. was developed. Pyraclostrobin were extracted from the samples with dichloromethane, cleaned up with SPE-C18 and analyzed by HPLC with C18 column, acetonitrile/water （70/30, by volume） as mobile phase and UV detector at 278nm. The results showed that the minimum detectable amount was 2xl0-1~g, the linear correlation was 0.998 6, the average recoveries were in the range of 80.4%-94.1% and 81.8%-98.4%, the RSD were 1.8%-3.8% and 2.6%-3.0% for grape and soil respectively. The method was sensitive, precise, rapid and simple. It was applicable to the determination of pyraclostrobin residues in grape and soil.
Pesticide Science and Administration