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葡萄和土壤中吡唑醚菌酯的高效液相色谱残留分析方法 预览 被引量:26

Determination of pyraclostrobin Residues in Grape and Soil by HPLC
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摘要 建立了测定葡萄及土壤中吡唑醚菌酯残留的液相色谱检测方法。样品以二氯甲烷提取和固相萃取柱(SPEC18柱)萃取净化,反向高效液相色谱法测定,乙腈和水(70+30)为流动相,C18色谱柱分离,278nm紫外定量检测吡唑醚菌酯。该方法吡唑醚菌酯的最小检出量为2×10^-10g,线性相关系数为0.9986,葡萄及土壤加标回收率分别为80.4%-94.1%、81.8%。98.4%.相对标准偏差(RSD)分别为1.8%~3.8%、2.6%-3.0%。方法具有简便、快捷。准确的特点,适合葡萄及土壤中吡唑醚菌酯残留量的测定。 A HPLC method for the determination of pyraclostrobin residues in grape and soil. was developed. Pyraclostrobin were extracted from the samples with dichloromethane, cleaned up with SPE-C18 and analyzed by HPLC with C18 column, acetonitrile/water (70/30, by volume) as mobile phase and UV detector at 278nm. The results showed that the minimum detectable amount was 2xl0-1~g, the linear correlation was 0.998 6, the average recoveries were in the range of 80.4%-94.1% and 81.8%-98.4%, the RSD were 1.8%-3.8% and 2.6%-3.0% for grape and soil respectively. The method was sensitive, precise, rapid and simple. It was applicable to the determination of pyraclostrobin residues in grape and soil.
作者 李瑞娟 于建垒 宋国春 Li Ruijuan, Yu Jianlei, Song Guochun (Plant Protection Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China)
出处 《农药科学与管理》 CAS 2010年第6期 33-36,共4页 Pesticide Science and Administration
基金 山东省资源节约型社会科技支撑体系建设专项计划(2007JY14)
关键词 吡唑醚菌酯 葡萄 土壤 液相色谱法 残留分析 pyraclostrobin grape soil HPLC residue analysis
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